Chapter title |
Solid-Phase Peptide-Carrier Conjugation
|
---|---|
Chapter number | 7 |
Book title |
Peptide Antibodies
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2999-3_7 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2998-6, 978-1-4939-2999-3
|
Authors |
Gunnar Houen, Dorthe T. Olsen, Houen, Gunnar, Olsen, Dorthe T. |
Abstract |
Conjugation to carrier proteins is necessary for peptides to be able to induce antibody formation when injected into animals together with a suitable adjuvant. This is usually performed by conjugation in solution followed by mixing with the adjuvant. Alternatively, the carrier may be adsorbed onto a solid support followed by activation and conjugation with the peptide by solid-phase chemistry. Different reagents can be used for conjugation through peptide functional groups (-SH, -NH2, -COOH) and various carrier proteins may be used depending on the peptides and the intended use of the antibodies. The solid phase may be an ion-exchange matrix, from which the conjugate can subsequently be eluted and mixed with adjuvant. Alternatively, the adjuvant aluminum hydroxide may be used as the solid-phase matrix, whereupon the carrier is immobilized and conjugated with peptide. The resulting adjuvant-carrier-peptide complexes may then be used directly for immunization. |
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