Chapter title |
Thioredoxin-Displayed Multipeptide Immunogens
|
---|---|
Chapter number | 14 |
Book title |
Peptide Antibodies
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2999-3_14 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2998-6, 978-1-4939-2999-3
|
Authors |
Bolchi, Angelo, Canali, Elena, Santoni, Andrea, Spagnoli, Gloria, Viarisio, Daniele, Accardi, Rosita, Tommasino, Massimo, Müller, Martin, Ottonello, Simone, Angelo Bolchi, Elena Canali, Andrea Santoni, Gloria Spagnoli, Daniele Viarisio, Rosita Accardi, Massimo Tommasino, Martin Müller, Simone Ottonello |
Abstract |
Fusion to carrier proteins is an effective strategy for stabilizing and providing immunogenicity to peptide epitopes. This is commonly achieved by cross-linking of chemically synthesized peptides to carrier proteins. An alternative approach is internal grafting of selected peptide epitopes to a scaffold protein via double stranded-oligonucleotide insertion or gene synthesis, followed by recombinant expression of the resulting chimeric polypeptide. The scaffold protein should confer immunogenicity to the stabilized and structurally constrained peptide, but also afford easy production of the antigen in recombinant form. A macromolecular scaffold that meets the above criteria is the redox protein thioredoxin, especially bacterial thioredoxin. Here we describe our current methodology for internal grafting of selected peptide epitopes to thioredoxin as tandemly arranged multipeptide repeats ("Thioredoxin Displayed Multipeptide Immunogens"), bacterial expression and purification of the recombinant thioredoxin-multipeptide fusion proteins and their use as antigens for the production of anti-peptide antibodies for prophylactic vaccine as well as diagnostic purposes. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 10 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Doctoral Student | 2 | 20% |
Student > Bachelor | 2 | 20% |
Student > Ph. D. Student | 2 | 20% |
Professor | 1 | 10% |
Other | 1 | 10% |
Other | 2 | 20% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 6 | 60% |
Agricultural and Biological Sciences | 2 | 20% |
Immunology and Microbiology | 1 | 10% |
Unknown | 1 | 10% |