Chapter title |
Parallel Syntheses of Peptides on Teflon-Patterned Paper Arrays (SyntArrays).
|
---|---|
Chapter number | 18 |
Book title |
Microarray Technology
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3136-1_18 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3135-4, 978-1-4939-3136-1
|
Authors |
Deiss, Frédérique, Yang, Yang, Derda, Ratmir, Frédérique Deiss, Yang Yang, Ratmir Derda |
Abstract |
Screening of peptides to find the ligands that bind to specific targets is an important step in drug discovery. These high-throughput screens require large number of structural variants of peptides to be synthesized and tested. This chapter describes the generation of arrays of peptides on Teflon-patterned sheets of paper. First, the protocol describes the patterning of paper with a Teflon solution to produce arrays with solvophobic barriers that are able to confine organic solvents. Next, we describe the parallel syntheses of 96 peptides on Teflon-patterned arrays using the SPOT synthesis method. |
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