Chapter title |
High-Throughput DNA Array for SNP Detection of KRAS Gene Using a Centrifugal Microfluidic Device.
|
---|---|
Chapter number | 10 |
Book title |
Microarray Technology
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3136-1_10 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3135-4, 978-1-4939-3136-1
|
Authors |
Sedighi, Abootaleb, Li, Paul C H, Abootaleb Sedighi, Paul C. H. Li |
Abstract |
Here, we describe detection of single nucleotide polymorphism (SNP) in genomic DNA samples using a NanoBioArray (NBA) chip. Fast DNA hybridization is achieved in the chip when target DNAs are introduced to the surface-arrayed probes using centrifugal force. Gold nanoparticles (AuNPs) are used to assist SNP detection at room temperature. The parallel setting of sample introduction in the spiral channels of the NBA chip enables multiple analyses on many samples, resulting in a technique appropriate for high-throughput SNP detection. The experimental procedure, including chip fabrication, probe array printing, DNA amplification, hybridization, signal detection, and data analysis, is described in detail. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Greece | 1 | 14% |
Unknown | 6 | 86% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 2 | 29% |
Student > Master | 2 | 29% |
Student > Postgraduate | 1 | 14% |
Unspecified | 1 | 14% |
Unknown | 1 | 14% |
Readers by discipline | Count | As % |
---|---|---|
Engineering | 2 | 29% |
Agricultural and Biological Sciences | 1 | 14% |
Unspecified | 1 | 14% |
Unknown | 3 | 43% |