| Chapter title |
Epitope Mapping Using Peptide Microarray in Autoantibody Profiling
|
|---|---|
| Chapter number | 15 |
| Book title |
Microarray Technology
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3136-1_15 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3135-4, 978-1-4939-3136-1
|
| Authors |
Sebastian Henkel, Robert Wellhausen, Dirk Woitalla, Katrin Marcus, Caroline May |
| Abstract |
The use of peptide microarrays for epitope mapping of autoantibodies greatly facilitates the early diagnosis of allergic, cytotoxin-associated diseases and especially inflammatory diseases. A common approach to create the microarrays utilizes nitrocellulose-coated glass slides for peptide probe binding, which is based on surface adsorption. Advantages of this method include excellent peptide binding capacity and long-term stability. To ensure equal accessibility to all antibodies on the peptide microarray during epitope mapping, all probes are immobilized in a random manner, thus avoiding concentration-dependent effects on signal intensity.In this chapter, we provide a step-by-step protocol on how to construct the peptide microarrays and perform epitope mapping of autoantibodies using them. Finally we present a comparative approach for the evaluation of the data. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 9 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Doctoral Student | 2 | 22% |
| Student > Master | 2 | 22% |
| Lecturer | 1 | 11% |
| Other | 1 | 11% |
| Researcher | 1 | 11% |
| Other | 0 | 0% |
| Unknown | 2 | 22% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 2 | 22% |
| Medicine and Dentistry | 2 | 22% |
| Chemical Engineering | 1 | 11% |
| Engineering | 1 | 11% |
| Unknown | 3 | 33% |