Chapter title |
Microarray Technology
|
---|---|
Chapter number | 13 |
Book title |
Microarray Technology
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3136-1_13 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3135-4, 978-1-4939-3136-1
|
Authors |
Gao, Liqian, Lee, Su Seong, Chen, Jun, Sun, Hongyan, Zhao, Yuliang, Chai, Zhifang, Hu, Yi, Liqian Gao, Su Seong Lee, Jun Chen, Hongyan Sun, Yuliang Zhao, Zhifang Chai, Yi Hu |
Abstract |
Phosphatases are a family of enzymes responsible for the dephosphorylation of biomolecules. Phosphatases play essential roles in cell cycle regulation, signal transduction, and cellular communication. In recent years, one type of phosphatases, protein tyrosine phosphatases (PTPs), emerges as important therapeutic targets for complex and devastating diseases. Nevertheless, the physiological roles, substrate specificity, and downstream targets for PTPs remain largely unknown. To demonstrate how microarrays can be applied to characterizing PTPs, we describe here a phosphopeptide microarray strategy for activity-based high-throughput screening of PTPs substrate specificity. This is followed by a kinetic microarray assay and microplate assay to determine the rate constants of dephosphorylation by PTPs. This microarray strategy has been successfully applied to identifying several potent and selective substrates against different PTPs. These substrates could be used to design potent and selective PTPs inhibitors in the future. |
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