Chapter title |
Methods to study tumor surveillance using tumor cell transplantation into genetically engineered mice.
|
---|---|
Chapter number | 22 |
Book title |
Mouse Models of Cancer
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2297-0_22 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2296-3, 978-1-4939-2297-0
|
Authors |
Eva Bauer, Agnieszka Witalisz, Birgit Strobl, Dagmar Stoiber, Bauer E, Witalisz A, Strobl B, Stoiber D, Bauer, Eva, Witalisz, Agnieszka, Strobl, Birgit, Stoiber, Dagmar |
Abstract |
When a tumor evolves, there is constant crosstalk between the transformed cells and cells of the immune system. Transplantation of well-established tumor cell lines into genetically engineered mice is a valuable tool to study the contribution of a gene of interest to tumor surveillance. These methods bear several advantages: first, such cell lines are well characterized; second, much data for reference exist; and third, the impact of the immune system can be separated from tumor cell intrinsic effects. Here, we provide protocols for tumor cell transplantations to address the role of a specific gene product in tumor surveillance. We furthermore describe several approaches to define the impact of natural killer cells and T cells, such as cell depletion and adoptive transfer experiments or use of different genetically modified mice. |
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Mendeley readers
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