Chapter title |
Extraction of RNA from cells and tissue.
|
---|---|
Book title |
Hypertension
|
Published in |
Methods in molecular medicine, July 2005
|
DOI | 10.1385/1-59259-850-1:139 |
Pubmed ID | |
Book ISBNs |
978-1-58829-323-7, 978-1-59259-850-2
|
Authors |
Bird IM, Bird M. Ian, Ian, Bird M. |
Abstract |
Total undegraded cellular RNA, largely free of contaminating DNA, can be rapidly and easily isolated from homogenized tissue or cultured cells using acid pH guanidinium thiocyanate/phenol/chloroform extraction. Commercially available acid phenol/guanidinium thiocyanate solutions allow one-step complete solubilization of tissue or cells under conditions that inhibit RNAse activity. We describe here a detailed procedure for total RNA extraction from homogenized tissues or cultured cells using such reagents, together with additional purification steps by passage through Phase-Lock gel tubes, and recommend optional chromatographic poly(A+) RNA enrichment procedure using Mini Oligo(dT) spin columns, together with the spectrophotometric determination of RNA yield and purity. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Hungary | 1 | 2% |
United States | 1 | 2% |
Unknown | 42 | 95% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 12 | 27% |
Student > Master | 8 | 18% |
Researcher | 6 | 14% |
Unspecified | 2 | 5% |
Professor > Associate Professor | 2 | 5% |
Other | 5 | 11% |
Unknown | 9 | 20% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 10 | 23% |
Agricultural and Biological Sciences | 9 | 20% |
Medicine and Dentistry | 4 | 9% |
Unspecified | 2 | 5% |
Veterinary Science and Veterinary Medicine | 2 | 5% |
Other | 7 | 16% |
Unknown | 10 | 23% |