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Protein Complex Assembly

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Cover of 'Protein Complex Assembly'

Table of Contents

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    Book Overview
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    Chapter 1 Experimental Characterization of Protein Complex Structure, Dynamics, and Assembly
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    Chapter 2 High-Throughput Electron Cryo-tomography of Protein Complexes and Their Assembly
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    Chapter 3 Preparation of Tunable Microchips to Visualize Native Protein Complexes for Single-Particle Electron Microscopy
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    Chapter 4 Time-Resolved Cryo-electron Microscopy Using a Microfluidic Chip
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    Chapter 5 Characterizing Protein-Protein Interactions Using Solution NMR Spectroscopy
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    Chapter 6 Reconstitution of Isotopically Labeled Ribosomal Protein L29 in the 50S Large Ribosomal Subunit for Solution-State and Solid-State NMR
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    Chapter 7 Characterizing Protein-Protein Interactions Using Deep Sequencing Coupled to Yeast Surface Display
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    Chapter 8 Structurally Guided In Vivo Crosslinking
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    Chapter 9 Characterizing Intact Macromolecular Complexes Using Native Mass Spectrometry
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    Chapter 10 Hydrogen-Deuterium Exchange Mass Spectrometry to Study Protein Complexes
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    Chapter 11 Structural Analysis of Protein Complexes by Cross-Linking and Mass Spectrometry
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    Chapter 12 Global Characterization of Protein Complexes by Biochemical Purification-Mass Spectrometry (BP/MS)
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    Chapter 13 Proteomic Profiling of Integrin Adhesion Complex Assembly
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    Chapter 14 Dual-Color and 3D Super-Resolution Microscopy of Multi-protein Assemblies
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    Chapter 15 Correlative 3D Structured Illumination Microscopy and Single-Molecule Localization Microscopy for Imaging Cancer Invasion
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    Chapter 16 Observing the Assembly of Protein Complexes in Living Eukaryotic Cells in Super-Resolution Using refSOFI
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    Chapter 17 Detecting Purinosome Metabolon Formation with Fluorescence Microscopy
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    Chapter 18 Analysis of Bacterial Pilus Assembly by Shearing and Immunofluorescence Microscopy
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    Chapter 19 Expression, Purification, and Assembly of Archaellum Subcomplexes of Sulfolobus acidocaldarius
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    Chapter 20 Unstable Protein Purification Through the Formation of Stable Complexes
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    Chapter 21 Expressing Multi-subunit Complexes Using biGBac
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    Chapter 22 Computational Modelling of Protein Complex Structure and Assembly
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    Chapter 23 Inferring and Using Protein Quaternary Structure Information from Crystallographic Data
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    Chapter 24 Searching and Extracting Data from the EMBL-EBI Complex Portal
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    Chapter 25 Automated Computational Inference of Multi-protein Assemblies from Biochemical Co-purification Data
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    Chapter 26 A Multiscale Computational Model for Simulating the Kinetics of Protein Complex Assembly
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    Chapter 27 Flexible Protein-Protein Docking with SwarmDock
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    Chapter 28 Protein-Protein Docking Using Evolutionary Information
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    Chapter 29 Modeling Structure and Dynamics of Protein Complexes with SAXS Profiles
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    Chapter 30 Modeling the Structure of Helical Assemblies with Experimental Constraints in Rosetta
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    Chapter 31 Selecting Conformational Ensembles Using Residual Electron and Anomalous Density (READ)
Attention for Chapter 30: Modeling the Structure of Helical Assemblies with Experimental Constraints in Rosetta
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Chapter title
Modeling the Structure of Helical Assemblies with Experimental Constraints in Rosetta
Chapter number 30
Book title
Protein Complex Assembly
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7759-8_30
Pubmed ID
Book ISBNs
978-1-4939-7758-1, 978-1-4939-7759-8
Authors

Ingemar André

Abstract

Determining high-resolution structures of proteins with helical symmetry can be challenging due to limitations in experimental data. In such instances, structure-based protein simulations driven by experimental data can provide a valuable approach for building models of helical assemblies. This chapter describes how the Rosetta macromolecular package can be used to model homomeric protein assemblies with helical symmetry in a range of modeling scenarios including energy refinement, symmetrical docking, comparative modeling, and de novo structure prediction. Data-guided structure modeling of helical assemblies with experimental information from electron density, X-ray fiber diffraction, solid-state NMR, and chemical cross-linking mass spectrometry is also described.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 7 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 5 71%
Professor > Associate Professor 1 14%
Student > Bachelor 1 14%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 3 43%
Chemistry 2 29%
Physics and Astronomy 1 14%
Engineering 1 14%