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Mendeley readers
Chapter title |
Characterizing Protein-Protein Interactions Using Solution NMR Spectroscopy
|
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Chapter number | 5 |
Book title |
Protein Complex Assembly
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Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7759-8_5 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7758-1, 978-1-4939-7759-8
|
Authors |
Jose Luis Ortega-Roldan, Martin Blackledge, Malene Ringkjøbing Jensen |
Abstract |
In this chapter, we describe how NMR chemical shift titrations can be used to study the interaction between two proteins with emphasis on mapping the interface of the complex and determining the binding affinity from a quantitative analysis of the experimental data. In particular, we discuss the appearance of NMR spectra in different chemical exchange regimes (fast, intermediate, and slow) and how these regimes affect NMR data analysis. |
Mendeley readers
The data shown below were compiled from readership statistics for 37 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 37 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 8 | 22% |
Student > Ph. D. Student | 5 | 14% |
Researcher | 5 | 14% |
Student > Doctoral Student | 4 | 11% |
Student > Master | 4 | 11% |
Other | 2 | 5% |
Unknown | 9 | 24% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 16 | 43% |
Agricultural and Biological Sciences | 4 | 11% |
Chemistry | 3 | 8% |
Medicine and Dentistry | 1 | 3% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 3% |
Other | 0 | 0% |
Unknown | 12 | 32% |