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Mendeley readers
| Chapter title |
Preparation of Tunable Microchips to Visualize Native Protein Complexes for Single-Particle Electron Microscopy
|
|---|---|
| Chapter number | 3 |
| Book title |
Protein Complex Assembly
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7759-8_3 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7758-1, 978-1-4939-7759-8
|
| Authors |
Brian L. Gilmore, A. Cameron Varano, William Dearnaley, Yanping Liang, Bridget C. Marcinkowski, Madeline J. Dukes, Deborah F. Kelly |
| Abstract |
Recent advances in technology have enabled single-particle electron microscopy (EM) to rapidly progress as a preferred tool to study protein assemblies. Newly developed materials and methods present viable alternatives to traditional EM specimen preparation. Improved lipid monolayer purification reagents offer considerable flexibility, while ultrathin silicon nitride films provide superior imaging properties to the structural study of protein complexes. Here, we describe the steps for combining monolayer purification with silicon nitride microchips to create a tunable approach for the EM community. |
Mendeley readers
The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 7 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 2 | 29% |
| Professor | 1 | 14% |
| Student > Master | 1 | 14% |
| Researcher | 1 | 14% |
| Student > Postgraduate | 1 | 14% |
| Other | 0 | 0% |
| Unknown | 1 | 14% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 4 | 57% |
| Physics and Astronomy | 1 | 14% |
| Unknown | 2 | 29% |