Chapter title |
Experimental Characterization of Protein Complex Structure, Dynamics, and Assembly
|
---|---|
Chapter number | 1 |
Book title |
Protein Complex Assembly
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7759-8_1 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7758-1, 978-1-4939-7759-8
|
Authors |
Jonathan N. Wells, Joseph A. Marsh, Wells, Jonathan N., Marsh, Joseph A. |
Abstract |
Experimental methods for the characterization of protein complexes have been instrumental in achieving our current understanding of the protein universe and continue to progress with each year that passes. In this chapter, we review some of the most important tools and techniques in the field, covering the important points in X-ray crystallography, cryo-electron microscopy, NMR spectroscopy, and mass spectrometry. Novel developments are making it possible to study large protein complexes at near-atomic resolutions, and we also now have the ability to study the dynamics and assembly pathways of protein complexes across a range of sizes. |
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Student > Bachelor | 3 | 21% |
Researcher | 2 | 14% |
Student > Ph. D. Student | 2 | 14% |
Student > Doctoral Student | 1 | 7% |
Lecturer > Senior Lecturer | 1 | 7% |
Other | 1 | 7% |
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Unknown | 4 | 29% |