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The Protein Protocols Handbook

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Cover of 'The Protein Protocols Handbook'

Table of Contents

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    Book Overview
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    Chapter 1 Protein Determination by UV Absorption
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    Chapter 2 The Lowry Method for Protein Quantitation
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    Chapter 3 The Bicinchoninic Acid (BCA) Assay for Protein Quantitation
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    Chapter 4 The Bradford Method for Protein Quantitation
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    Chapter 5 Ultrafast Protein Determinations Using Microwave Enhancement
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    Chapter 6 Quantitation of Tryptophan in Proteins
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    Chapter 7 Protein Quantitation Using Flow Cytometry
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    Chapter 8 Copper Iodide Staining of Proteins on Solid Phases
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    Chapter 9 Kinetic Silver Staining of Proteins Adsorbed to Microtiter Plates
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    Chapter 10 Nondenaturing Polyacrylamide Gel Electrophoresis of Proteins
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    Chapter 11 SDS Polyacrylamide Gel Electrophoresis of Proteins
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    Chapter 12 Gradient SDS Polyacrylamide Gel Electrophoresis of Proteins
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    Chapter 13 Cetyltrimethylammonium Bromide Discontinuous Gel Electrophoresis of Proteins
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    Chapter 14 Acetic Acid-Urea Polyacrylamide Gel Electrophoresis of Basic Proteins
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    Chapter 15 Acid-Urea-Triton Polyacrylamide Gels for Histones
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    Chapter 16 SDS-Polyacrylamide Gel Electrophoresis of Peptides
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    Chapter 17 Isoelectric Focusing of Proteins in Ultra-Thin Polyacrylamide Gels
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    Chapter 18 Isoelectric Focusing Under Denaturing Conditions
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    Chapter 19 Radioisotopic Labeling of Proteins for Polyacrylamide Gel Electrophoresis
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    Chapter 20 Two-Dimensional PAGE Using Carrier Ampholyte pH Gradients in the First Dimension
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    Chapter 21 Two-Dimensional PAGE Using Immobilized pH Gradients
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    Chapter 22 Two-Dimensional PAGE Using Flat-Bed IEF in the First Dimension
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    Chapter 23 Free Zone Capillary Electrophoresis
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    Chapter 24 Capillary Isoelectric Focusing with Electro-Osmotic Flow Mobilization
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    Chapter 25 Quantification of Radiolabeled Proteins in Polyacrylamide Gels
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    Chapter 26 Quantification of Proteins by Staining in Polyacrylamide Gels
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    Chapter 27 Quantitation of Proteins Separated by Electrophoresis Using Coomassie Brilliant Blue
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    Chapter 28 Rapid Staining of Proteins in Polyacrylamide Gels with Nile Red
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    Chapter 29 Zn 2+ -Reverse Staining of Proteins in Polyacrylamide Gels
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    Chapter 30 Protein Staining with Calconcarboxylic Acid in Polyacrylamide Gels
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    Chapter 31 Detection of Proteins and Sialoglycoproteins in Polyacrylamide Gels Using Eosin Y Stain
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    Chapter 32 Electroelution of Proteins from Polyacrylamide Gels
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    Chapter 33 High-Performance Electrophoresis Chromatography
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    Chapter 34 Drying Polyacrylamide Gels
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    Chapter 35 Detection of Proteins in Polyacrylamide Gels by Silver Staining
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    Chapter 36 Autoradiography and Fluorography of Acrylamide Gels
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    Chapter 37 Protein Blotting by Electroblotting
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    Chapter 38 Protein Blotting by the Semidry Method
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    Chapter 39 Protein Blotting by the Capillary Method
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    Chapter 40 Protein Blotting of Basic Proteins Resolved on Acid-Urea-Triton-Polyacrylamide Gels
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    Chapter 41 Alkaline Phosphatase Labeling of Antibody Using Glutaraldehyde
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    Chapter 42 β-Galactosidase Labeling of Antibody Using MBS
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    Chapter 43 Horseradish Peroxidase Labeling of Antibody Using Periodate Oxidation
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    Chapter 44 Protein Staining and Immunodetection Using Colloidal Gold
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    Chapter 45 Fluorescent Protein Staining on Nitrocellulose with Subsequent Immunodetection of Antigen
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    Chapter 46 Coupling of Antibodies with Biotin
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    Chapter 47 Preparation of Avidin Conjugates
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    Chapter 48 Detection of Polypeptides on Blots Using Secondary Antibodies or Protein A
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    Chapter 49 Detection of Proteins on Blots Using Avidin- or Streptavidin-Biotin
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    Chapter 50 Detection of Proteins on Blots Using Chemiluminescent Systems
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    Chapter 51 Carboxymethylation of Cysteine Using Iodoacetamide/Iodoacetic Acid
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    Chapter 52 Performic Acid Oxidation
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    Chapter 53 Succinylation of Proteins
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    Chapter 54 Pyridylethylation of Cysteine Residues
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    Chapter 55 Side-Chain Selective Chemical Modifications of Proteins
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    Chapter 56 Nitration of Tyrosines
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    Chapter 57 Ethoxyformylation of Histidine
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    Chapter 58 Modification of Arginine Side Chains with p -Hydroxyphenylglyoxal
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    Chapter 59 Amidation of Carboxyl Groups
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    Chapter 60 Amidination of Lysine Side Chains
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    Chapter 61 Modification of Tryptophan with 2-Hydroxy-5-Nitrobenzylbromide
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    Chapter 62 Modification of Sulfhydryl Groups with DTNB
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    Chapter 63 Chemical Cleavage of Proteins at Methionyl Residues
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    Chapter 64 Chemical Cleavage of Proteins at Tryptophan Residues
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    Chapter 65 Chemical Cleavage of Proteins at Aspartyl Residues
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    Chapter 66 Chemical Cleavage of Proteins at Cysteinyl Residues
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    Chapter 67 Chemical Cleavage of Proteins at Asparaginyl-Glycyl Peptide Bonds
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    Chapter 68 Preparation of Peptides for Microsequencing from Proteins in Polyacrylamide Gels
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    Chapter 69 In Situ Chemical and Enzymatic Digestions of Proteins Immobilized on Miniature Hydrophobic Columns
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    Chapter 70 Enzymatic Digestion of Membrane-Bound Proteins for Peptide Mapping and Internal Sequence Analysis
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    Chapter 71 Enzymatic Digestion of Proteins in Solution and in SDS Polyacrylamide Gels
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    Chapter 72 Reverse-Phase HPLC Separation of Enzymatic Digests of Proteins
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    Chapter 73 Peptide Mapping by Two-Dimensional Thin-Layer Electrophoresis-Thin-Layer Chromatography
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    Chapter 74 Peptide Mapping by Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis
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    Chapter 75 Peptide Mapping by High-Performance Liquid Chromatography
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    Chapter 76 Production of Protein Hydrolysates Using Enzymes
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    Chapter 77 Amino Acid Analysis Using Precolumn Derivatization with 6-Aminoquinolyl- n -Hydroxysuccinimidyl Carbamate
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    Chapter 78 Amino Acid Analysis Using Precolumn Derivatization with Phenylisothiocyanate
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    Chapter 79 Molecular-Weight Estimation for Native Proteins Using Size-Exclusion High-Performance Liquid Chromatography
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    Chapter 80 Detection of Disulfide-Linked Peptides by HPLC
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    Chapter 81 Diagonal Electrophoresis for Detecting Disulfide Bridges
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    Chapter 82 Estimation of Disulfide Bonds Using Ellman’s Reagent
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    Chapter 83 Quantitation of Cysteine Residues and Disulfide Bonds by Electrophoresis
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    Chapter 84 Detection of Disulfide-Linked Peptides by Mass Spectrometry
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    Chapter 85 Analyzing Protein Phosphorylation
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    Chapter 86 Identification of Proteins Modified by Protein ( D -Aspartyl/ L -Isoaspartyl) Carboxyl Methyltransferase
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    Chapter 87 Analysis of Protein Palmitoylation
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    Chapter 88 Removal of Pyroglutamic Acid Residues from the N-Terminus of Peptides and Proteins
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    Chapter 89 The Dansyl Method for Identifying N-Terminal Amino Acids
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    Chapter 90 The Dansyl-Edman Method for Peptide Sequencing
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    Chapter 91 Matrix-Assisted Laser Desorption Ionization Mass Spectrometry as a Complement to Internal Protein Sequencing
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    Chapter 92 A Manual C-Terminal Sequencing Procedure for Peptides
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    Chapter 93 C-Terminal Sequence Analysis with Carboxypeptidase Y
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    Chapter 94 Rapid Epitope Mapping by Carboxypeptidase Digestion and Immunoblotting
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    Chapter 95 Epitope Mapping of a Protein Using the Geysen (PEPSCAN) Procedure
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    Chapter 96 Epitope Mapping of Protein Antigens by Competition ELISA
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    Chapter 97 Identification of Glycoproteins on Nitrocellulose Membranes Using Lectin Blotting
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    Chapter 98 A Lectin-Binding Assay for the Rapid Characterization of the Glycosylation of Purified Glycoproteins
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    Chapter 99 Staining of Glycoproteins/Proteoglycans on SDS-Gels
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    Chapter 100 Chemical Methods of Analysis of Glycoproteins
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    Chapter 101 Monosaccharide Analysis by HPAEC
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    Chapter 102 Monosaccharide Analysis by GC
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    Chapter 103 Determination of Monosaccharide Linkage and Substitution Patterns by GC-MS Methyiation Analysis
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    Chapter 104 Sialic Acid Analysis by HPAEC-PAD
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    Chapter 105 Chemical Release of O -Linked Oligosaccharide Chains
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    Chapter 106 O -Linked Oligosaccharide Profiling by HPLC
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    Chapter 107 O -Linked Oligosaccharide Profiling by HPAEC-PAD
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    Chapter 108 Release of N -Linked Oligosaccharide Chains by Hydrazinolysis
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    Chapter 109 Enzymatic Release of O - and N -Linked Oligosaccharide Chains
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    Chapter 110 N -Linked Oligosaccharide Profiling by HPLC on Porous Graphitized Carbon (PGC)
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    Chapter 111 N -Linked Oligosaccharide Profiling by HPAEC-PAD
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    Chapter 112 The Chloramine T Method for Radiolabeling Protein
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    Chapter 113 The Lactoperoxidase Method for Radiolabeling Protein
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    Chapter 114 The Bolton and Hunter Method for Radiolabeling Protein
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    Chapter 115 The Iodogen Method for Radiolabeling Protein
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    Chapter 116 Purification and Assessment of Quality of Radioiodinated Protein
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    Chapter 117 Conjugation of Peptides to Carrier Proteins via Glutaraldehyde
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    Chapter 118 Conjugation of Peptide to Carrier Proteins via m -Maleimidobenzoyl- N -Hydroxysuccinimide Ester (MBS)
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    Chapter 119 Conjugation of Peptides to Carrier Protein via Carbodiimide
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    Chapter 120 Raising of Polyclonal Antisera
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    Chapter 121 Elution of SDS-PAGE Separated Proteins from Immobilon Membranes for Use as Antigens
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    Chapter 122 Production of Highly Specific Polyclonal Antibodies Using a Combination of 2D Electrophoresis and Nitrocellulose-Bound Antigen
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    Chapter 123 Production and Characterization of Antibodies Against Peptides
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    Chapter 124 Production of Antibodies Using Proteins in Gel Bands
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    Chapter 125 Purification of IgG by Precipitation with Sodium Sulfate or Ammonium Sulfate
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    Chapter 126 Purification of IgG Using Caprylic Acid
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    Chapter 127 Purification of IgG Using DEAE-Sepharose Chromatography
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    Chapter 128 Purification of IgG Using Ion-Exchange HPLC/FPLC
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    Chapter 129 Purification of IgG by Precipitation with Polyethylene Glycol (PEG)
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    Chapter 130 Purification of IgG Using Protein A or Protein G
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    Chapter 131 Purification of IgG Using Gel-Filtration Chromatography
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    Chapter 132 Purification of IgG Using Affinity Chromatography on Antigen-Ligand Columns
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    Chapter 133 Purification of IgG Using Thiophilic Chromatography
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    Chapter 134 Analysis of IgG Fractions by Electrophoresis
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    Chapter 135 The Protein Protocols Handbook
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    Chapter 136 Single Radial Immunodiffusion
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    Chapter 137 Rocket Immunoelectrophoresis
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    Chapter 138 Two-Dimensional Immunoelectrophoresis
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    Chapter 139 Immunogen Preparation and Immunization Procedures for Rats and Mice
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    Chapter 140 Hybridoma Production
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    Chapter 141 Screening Hybridoma Culture Supernatants Using Solid-Phase Radiobinding Assay
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    Chapter 142 Screening Hybridoma Culture Supernatants Using ELISA
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    Chapter 143 Growth and Purification of Murine Monoclonal Antibodies
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    Chapter 144 A Rapid Method for Generating Large Numbers of High-Affinity Monoclonal Antibodies from a Single Mouse
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Mentioned by

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37 patents
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3 Wikipedia pages

Citations

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222 Dimensions

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41 Mendeley
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Title
The Protein Protocols Handbook
Published by
Humana Press, February 2008
DOI 10.1007/978-1-60327-259-9
ISBNs
978-0-89603-338-2, 978-1-60327-259-9
Editors

John M. Walker

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 41 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Germany 1 2%
Unknown 40 98%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 10 24%
Student > Master 9 22%
Student > Ph. D. Student 6 15%
Other 2 5%
Student > Postgraduate 2 5%
Other 6 15%
Unknown 6 15%
Readers by discipline Count As %
Agricultural and Biological Sciences 11 27%
Biochemistry, Genetics and Molecular Biology 6 15%
Medicine and Dentistry 3 7%
Chemistry 3 7%
Immunology and Microbiology 2 5%
Other 8 20%
Unknown 8 20%