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PCR Protocols

Overview of attention for book
Cover of 'PCR Protocols'

Table of Contents

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    Book Overview
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    Chapter 1 Setup of a PCR Laboratory
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    Chapter 2 Long-Range PCR with a DNA Polymerase Fusion
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    Chapter 3 Isolation of Genomic Insertion Sites of Proviruses Using Splinkerette-PCR-Based Procedures
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    Chapter 4 Lariat-Dependent Nested PCR for Flanking Sequence Determination
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    Chapter 5 CODEHOP PCR and CODEHOP PCR Primer Design
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    Chapter 6 Sequencing of Difficult DNA Regions by SAM Sequencing
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    Chapter 7 A Global Single-Cell cDNA Amplification Method for Quantitative Microarray Analysis
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    Chapter 8 Quantitation of microRNAs by real-time RT-qPCR.
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    Chapter 9 High-Throughput SuperSAGE
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    Chapter 10 Deep Cap Analysis of Gene Expression
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    Chapter 11 Linking Emulsion PCR Haplotype Analysis
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    Chapter 12 PAP-LMPCR: An Improved, Sequence-Selective Method for the In Vivo Analysis of Transcription Factor Occupancy and Chromatin Fine Structure
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    Chapter 13 The Many Faces of MLPA
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    Chapter 14 PCR Protocols
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    Chapter 15 Alu PCR.
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    Chapter 16 Asynchronous PCR
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    Chapter 17 Novel Applications of PCR Through the Use of DNA Substrates
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    Chapter 18 PCR Protocols
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    Chapter 19 Application of Blocking Oligonucleotides to Improve Signal-to-Noise Ratio in a PCR
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    Chapter 20 Asymmetric Overlap Extension PCR Method for Site-Directed Mutagenesis
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    Chapter 21 Ribosome Display: A Technology for Selecting and Evolving Proteins from Large Libraries
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    Chapter 22 GLOBE: Analysis of DNA–Protein Interaction Analysis
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    Chapter 23 PCR DNA-Array Profiling of DNA-Binding Transcription Factor Activities in Adult Mouse Tissues
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    Chapter 24 Nucleotide Exchange and Excision Technology DNA Shuffling and Directed Evolution
Attention for Chapter 15: Alu PCR.
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Chapter title
Alu PCR.
Chapter number 15
Book title
PCR Protocols
Published in
Methods in molecular biology, October 2010
DOI 10.1007/978-1-60761-944-4_15
Pubmed ID
Book ISBNs
978-1-60761-943-7, 978-1-60761-944-4
Authors

Maurizio Cardelli

Abstract

Alu PCR is a rapid and easy-to-perform "DNA fingerprinting" technique based on the simultaneous analysis of many genomic loci flanked by Alu repetitive elements, which allows the detection of genetic polymorphisms and mutations in human and primate genomes. In the protocol described in the present chapter, two fluorochrome-labelled primers complementary to Alu sequences are used to perform the PCR, and the amplification products are then analysed by capillary electrophoresis. The resulting -complex electrophoretic pattern may show sample-to-sample variability due to insertion, deletion, or sequence change of Alu retrotransposons, or caused by length variation of the sequence interposed between two Alus.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 26 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Canada 1 4%
Unknown 25 96%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 7 27%
Student > Master 7 27%
Student > Ph. D. Student 4 15%
Researcher 4 15%
Student > Postgraduate 1 4%
Other 0 0%
Unknown 3 12%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 15 58%
Agricultural and Biological Sciences 5 19%
Nursing and Health Professions 1 4%
Immunology and Microbiology 1 4%
Unknown 4 15%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 October 2013.
All research outputs
#7,453,827
of 22,787,797 outputs
Outputs from Methods in molecular biology
#2,316
of 13,094 outputs
Outputs of similar age
#35,518
of 99,610 outputs
Outputs of similar age from Methods in molecular biology
#8
of 23 outputs
Altmetric has tracked 22,787,797 research outputs across all sources so far. This one is in the 44th percentile – i.e., 44% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,094 research outputs from this source. They receive a mean Attention Score of 3.4. This one has done well, scoring higher than 76% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 99,610 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 24th percentile – i.e., 24% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 23 others from the same source and published within six weeks on either side of this one. This one is in the 43rd percentile – i.e., 43% of its contemporaries scored the same or lower than it.