| Chapter title |
Using the Zebrafish Embryo to Dissect the Early Steps of the Metastasis Cascade
|
|---|---|
| Chapter number | 15 |
| Book title |
Cell Migration
|
| Published in |
Methods in molecular biology, March 2018
|
| DOI | 10.1007/978-1-4939-7701-7_15 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7700-0, 978-1-4939-7701-7
|
| Authors |
Gautier Follain, Naël Osmani, Cédric Fuchs, Guillaume Allio, Sébastien Harlepp, Jacky G. Goetz |
| Abstract |
Most cancers end up with the death of patients caused by the formation of secondary tumors, called metastases. However, how these secondary tumors appear and develop is only poorly understood. A fine understanding of the multiple steps of the metastasis cascade requires in vivo models allowing high spatiotemporal analysis of the behavior of metastatic cells. Zebrafish embryos combine several advantages such as transparency, small size, stereotyped anatomy, and easy handling, making it a very powerful model for cell and cancer biology, and in vivo imaging analysis. In the following chapter, we describe a complete procedure allowing in vivo imaging methods, at high throughput and spatiotemporal resolution, to assess the behavior of circulating tumor cells (CTCs) in an experimental metastasis assay. This protocol provides access, for the first time, to the earliest steps of tumor cell seeding during metastasis formation. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 25 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 5 | 20% |
| Other | 4 | 16% |
| Professor > Associate Professor | 4 | 16% |
| Student > Master | 4 | 16% |
| Student > Doctoral Student | 2 | 8% |
| Other | 2 | 8% |
| Unknown | 4 | 16% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 8 | 32% |
| Agricultural and Biological Sciences | 2 | 8% |
| Medicine and Dentistry | 2 | 8% |
| Engineering | 2 | 8% |
| Immunology and Microbiology | 1 | 4% |
| Other | 1 | 4% |
| Unknown | 9 | 36% |