| Chapter title |
Methods to assay inhibitors of DNA gyrase and topoisomerase IV activities.
|
|---|---|
| Chapter number | 2 |
| Book title |
New Antibiotic Targets
|
| Published in |
Methods in molecular medicine, April 2008
|
| DOI | 10.1007/978-1-59745-246-5_2 |
| Pubmed ID | |
| Book ISBNs |
978-1-58829-915-4, 978-1-59745-246-5
|
| Authors |
L. Mark Fisher, Xiao-Su Pan, Fisher, L. Mark, Pan, Xiao-Su |
| Abstract |
DNA gyrase and DNA topoisomerase (topo) IV are the bacterial targets of coumarin and quinolone antimicrobial agents. Widespread resistance to clinically important antibiotics such as beta-lactams and macrolides has stimulated the development of novel gyrase and topo IV inhibitors especially against Streptococcus pneumoniae and other Gram-positive pathogens. Here, we describe how gyrase and topo IV activities are measured and how inhibitors of these enzymes may be assayed, focusing as a paradigm on DNA supercoiling by S. pneumoniae gyrase, DNA decatenation by S. pneumoniae topo IV, and DNA cleavage by both enzymes. These approaches provide mechanistic insight on inhibitor action and allow identification of dual gyrase/topo IV targeting agents that can minimize the emergence of bacterial resistance. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 42 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 8 | 19% |
| Student > Bachelor | 7 | 17% |
| Student > Ph. D. Student | 7 | 17% |
| Other | 3 | 7% |
| Professor > Associate Professor | 3 | 7% |
| Other | 6 | 14% |
| Unknown | 8 | 19% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 12 | 29% |
| Chemistry | 7 | 17% |
| Biochemistry, Genetics and Molecular Biology | 7 | 17% |
| Medicine and Dentistry | 2 | 5% |
| Chemical Engineering | 1 | 2% |
| Other | 5 | 12% |
| Unknown | 8 | 19% |