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Mendeley readers
Chapter title |
Assessing Mitochondrial Bioenergetics by Respirometry in Cells or Isolated Organelles
|
---|---|
Chapter number | 18 |
Book title |
AMPK
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7598-3_18 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7597-6, 978-1-4939-7598-3
|
Authors |
Guillaume Vial, Bruno Guigas |
Abstract |
Mitochondrial oxidative phosphorylation is central for generating ATP and maintaining energy homeostasis in most eukaryotic cells. The ex vivo measurement of mitochondrial oxygen consumption rates in intact cells or isolated organelles is a valuable approach to assess mitochondrial bioenergetics in various experimental conditions. In this chapter, we describe several step-by-step protocols for measuring mitochondrial respiration in intact cells, permeabilized cells (in situ mitochondria), and isolated organelles using both Clark-type polarographic oxygen electrode devices and the newly developed oxygen-sensing fluorophore-based Seahorse technology. |
Mendeley readers
The data shown below were compiled from readership statistics for 20 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 20 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Doctoral Student | 4 | 20% |
Student > Master | 3 | 15% |
Student > Ph. D. Student | 3 | 15% |
Student > Bachelor | 2 | 10% |
Lecturer | 1 | 5% |
Other | 2 | 10% |
Unknown | 5 | 25% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 7 | 35% |
Medicine and Dentistry | 3 | 15% |
Agricultural and Biological Sciences | 2 | 10% |
Decision Sciences | 1 | 5% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 5% |
Other | 0 | 0% |
Unknown | 6 | 30% |