Chapter title |
Study of AMPK-Regulated Metabolic Fluxes in Neurons Using the Seahorse XFe Analyzer
|
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Chapter number | 19 |
Book title |
AMPK
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7598-3_19 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7597-6, 978-1-4939-7598-3
|
Authors |
Claudia Marinangeli, Jérome Kluza, Philippe Marchetti, Luc Buée, Valérie Vingtdeux |
Abstract |
AMP-activated protein kinase (AMPK) is the intracellular master energy sensor and metabolic regulator. AMPK is involved in cell energy homeostasis through the regulation of glycolytic flux and mitochondrial biogenesis. Interestingly, metabolic dysfunctions and AMPK deregulations are observed in many neurodegenerative diseases, including Alzheimer's. While these deregulations could play a key role in the development of these diseases, the study of metabolic fluxes has remained quite challenging and time-consuming. In this chapter, we describe the Seahorse XFe respirometry assay as a fundamental experimental tool to investigate the role of AMPK in controlling and modulating cell metabolic fluxes in living and intact differentiated primary neurons. The Seahorse XFe respirometry assay allows the real-time monitoring of glycolytic flux and mitochondrial respiration from different kind of cells, tissues, and isolated mitochondria. Here, we specify a protocol optimized for primary neuronal cells using several energy substrates such as glucose, pyruvate, lactate, glutamine, and ketone bodies. Nevertheless, this protocol can easily be adapted to monitor metabolic fluxes from other types of cells, tissues, or isolated mitochondria by taking into account the notes proposed for each key step of this assay. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 25 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 5 | 20% |
Researcher | 4 | 16% |
Student > Master | 3 | 12% |
Student > Postgraduate | 2 | 8% |
Student > Bachelor | 2 | 8% |
Other | 3 | 12% |
Unknown | 6 | 24% |
Readers by discipline | Count | As % |
---|---|---|
Neuroscience | 7 | 28% |
Biochemistry, Genetics and Molecular Biology | 4 | 16% |
Agricultural and Biological Sciences | 2 | 8% |
Medicine and Dentistry | 2 | 8% |
Psychology | 1 | 4% |
Other | 2 | 8% |
Unknown | 7 | 28% |