↓ Skip to main content

AMPK

Overview of attention for book
Cover of 'AMPK'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Production and Crystallization of Full-Length Human AMP-Activated Protein Kinase (α1β1γ1)
  3. Altmetric Badge
    Chapter 2 Visualizing AMPK Drug Binding Sites Through Crystallization of Full-Length Phosphorylated α2β1γ1 Heterotrimer
  4. Altmetric Badge
    Chapter 3 Biophysical Interactions of Direct AMPK Activators
  5. Altmetric Badge
    Chapter 4 Biochemical Measurement of Glycogen: Method to Investigate the AMPK-Glycogen Relationship
  6. Altmetric Badge
    Chapter 5 Cell-Free Assays to Measure Effects of Regulatory Ligands on AMPK
  7. Altmetric Badge
    Chapter 6 Applications of NMR and ITC for the Study of the Kinetics of Carbohydrate Binding by AMPK β-Subunit Carbohydrate-Binding Modules
  8. Altmetric Badge
    Chapter 7 Bioinformatics Approach to Identify Novel AMPK Targets
  9. Altmetric Badge
    Chapter 8 Studying AMPK in an Evolutionary Context
  10. Altmetric Badge
    Chapter 9 AMPK Protein Interaction Analyses by Yeast Two-Hybrid
  11. Altmetric Badge
    Chapter 10 Transient Expression of AMPK Heterotrimer Complexes in Mammalian Cells
  12. Altmetric Badge
    Chapter 11 Knockdown of Human AMPK Using the CRISPR/Cas9 Genome-Editing System
  13. Altmetric Badge
    Chapter 12 Compound C/Dorsomorphin: Its Use and Misuse as an AMPK Inhibitor
  14. Altmetric Badge
    Chapter 13 Identifying the Heterotrimeric Complex Stoichiometry of AMPK in Skeletal Muscle by Immunoprecipitation
  15. Altmetric Badge
    Chapter 14 Kinase Activity Determination of Specific AMPK Complexes/Heterotrimers in the Skeletal Muscle
  16. Altmetric Badge
    Chapter 15 Determination of Adenine Nucleotide Concentrations in Cells and Tissues by High-Performance Liquid Chromatography
  17. Altmetric Badge
    Chapter 16 Intact Cell Assays to Monitor AMPK and Determine the Contribution of the AMP-Binding or ADaM Sites to Activation
  18. Altmetric Badge
    Chapter 17 Cellular Application of Genetically Encoded Sensors and Impeders of AMPK
  19. Altmetric Badge
    Chapter 18 Assessing Mitochondrial Bioenergetics by Respirometry in Cells or Isolated Organelles
  20. Altmetric Badge
    Chapter 19 Study of AMPK-Regulated Metabolic Fluxes in Neurons Using the Seahorse XFe Analyzer
  21. Altmetric Badge
    Chapter 20 Investigating the Role of AMPK in Inflammation
  22. Altmetric Badge
    Chapter 21 Studying the Role of AMPK in Cardiac Hypertrophy and Protein Synthesis
  23. Altmetric Badge
    Chapter 22 Assessment of AMPK-Stimulated Cellular Long-Chain Fatty Acid and Glucose Uptake
  24. Altmetric Badge
    Chapter 23 Measurement of AMPK-Induced Inhibition of Lipid Synthesis Flux in Cultured Cells
  25. Altmetric Badge
    Chapter 24 Studying the Role of AMPK in Autophagy
  26. Altmetric Badge
    Chapter 25 Determining AMPK Activation via the Lysosomal v-ATPase-Ragulator-AXIN/LKB1 Axis
  27. Altmetric Badge
    Chapter 26 Manipulation and Measurement of AMPK Activity in Pancreatic Islets
  28. Altmetric Badge
    Chapter 27 Analyzing AMPK Function in the Hypothalamus
  29. Altmetric Badge
    Chapter 28 Using Ex Vivo Kidney Slices to Study AMPK Effects on Kidney Proteins
  30. Altmetric Badge
    Chapter 29 A Flow Cytometry-Based Protocol to Measure Lymphocyte Viability Upon Metabolic Stress
  31. Altmetric Badge
    Chapter 30 Methods to Evaluate AMPK Regulation of Macrophage Cholesterol Homeostasis
  32. Altmetric Badge
    Chapter 31 Modulation of Vascular Function by AMPK: Assessment of NO Bioavailability and Surrogates of Oxidative Stress
  33. Altmetric Badge
    Chapter 32 Measurement of Reactive Oxygen Species (ROS) and Mitochondrial ROS in AMPK Knockout Mice Blood Vessels
  34. Altmetric Badge
    Chapter 33 Studying the Role of AMPK in Angiogenesis
  35. Altmetric Badge
    Chapter 34 Analysis of Muscle Stem Cell Fate Through Modulation of AMPK Activity
  36. Altmetric Badge
    Chapter 35 Evaluating the Role of Host AMPK in Leishmania Burden
  37. Altmetric Badge
    Chapter 36 Analysis of Transgenerational Phenotypes Following Acute Starvation in AMPK-Deficient C. elegans
  38. Altmetric Badge
    Chapter 37 Human γ2-AMPK Mutations
Attention for Chapter 25: Determining AMPK Activation via the Lysosomal v-ATPase-Ragulator-AXIN/LKB1 Axis
Altmetric Badge

Citations

dimensions_citation
5 Dimensions

Readers on

mendeley
14 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Chapter title
Determining AMPK Activation via the Lysosomal v-ATPase-Ragulator-AXIN/LKB1 Axis
Chapter number 25
Book title
AMPK
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7598-3_25
Pubmed ID
Book ISBNs
978-1-4939-7597-6, 978-1-4939-7598-3
Authors

Chen-Song Zhang, Mengqi Li, Yue Zong, Sheng-Cai Lin

Abstract

Recent studies have revealed how AMPK is activated inside the cell and animal tissues: in response to low glucose, AXIN tethers LKB1, by virtue of their constitutive association, to AMPK located on the surface of late endosome/lysosome. Importantly, the lysosomal v-ATPase (vacuolar ATPase)-Ragulator complex, when primed by glucose starvation or concanamycin A, facilitates AXIN/LKB1 to interact with AMPK. Here, we describe the experimental procedures of the assays for detecting the translocation of AXIN/LKB1 or the assembly of the AXIN-based AMPK-activating complexes on the late endosome/lysosome. The methods in this chapter will be useful for determining whether various metabolic stresses or pharmacological stimuli activate AMPK via the v-ATPase-Ragulator-AXIN/LKB1 axis, which also concomitantly inactivates mTORC1. Detailed protocols for determining the levels of adenylates are also described.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 14 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 14 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 2 14%
Student > Ph. D. Student 2 14%
Professor 1 7%
Student > Master 1 7%
Researcher 1 7%
Other 1 7%
Unknown 6 43%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 5 36%
Agricultural and Biological Sciences 2 14%
Medicine and Dentistry 1 7%
Unknown 6 43%