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AMPK

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Cover of 'AMPK'

Table of Contents

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    Book Overview
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    Chapter 1 Production and Crystallization of Full-Length Human AMP-Activated Protein Kinase (α1β1γ1)
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    Chapter 2 Visualizing AMPK Drug Binding Sites Through Crystallization of Full-Length Phosphorylated α2β1γ1 Heterotrimer
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    Chapter 3 Biophysical Interactions of Direct AMPK Activators
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    Chapter 4 Biochemical Measurement of Glycogen: Method to Investigate the AMPK-Glycogen Relationship
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    Chapter 5 Cell-Free Assays to Measure Effects of Regulatory Ligands on AMPK
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    Chapter 6 Applications of NMR and ITC for the Study of the Kinetics of Carbohydrate Binding by AMPK β-Subunit Carbohydrate-Binding Modules
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    Chapter 7 Bioinformatics Approach to Identify Novel AMPK Targets
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    Chapter 8 Studying AMPK in an Evolutionary Context
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    Chapter 9 AMPK Protein Interaction Analyses by Yeast Two-Hybrid
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    Chapter 10 Transient Expression of AMPK Heterotrimer Complexes in Mammalian Cells
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    Chapter 11 Knockdown of Human AMPK Using the CRISPR/Cas9 Genome-Editing System
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    Chapter 12 Compound C/Dorsomorphin: Its Use and Misuse as an AMPK Inhibitor
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    Chapter 13 Identifying the Heterotrimeric Complex Stoichiometry of AMPK in Skeletal Muscle by Immunoprecipitation
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    Chapter 14 Kinase Activity Determination of Specific AMPK Complexes/Heterotrimers in the Skeletal Muscle
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    Chapter 15 Determination of Adenine Nucleotide Concentrations in Cells and Tissues by High-Performance Liquid Chromatography
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    Chapter 16 Intact Cell Assays to Monitor AMPK and Determine the Contribution of the AMP-Binding or ADaM Sites to Activation
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    Chapter 17 Cellular Application of Genetically Encoded Sensors and Impeders of AMPK
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    Chapter 18 Assessing Mitochondrial Bioenergetics by Respirometry in Cells or Isolated Organelles
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    Chapter 19 Study of AMPK-Regulated Metabolic Fluxes in Neurons Using the Seahorse XFe Analyzer
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    Chapter 20 Investigating the Role of AMPK in Inflammation
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    Chapter 21 Studying the Role of AMPK in Cardiac Hypertrophy and Protein Synthesis
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    Chapter 22 Assessment of AMPK-Stimulated Cellular Long-Chain Fatty Acid and Glucose Uptake
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    Chapter 23 Measurement of AMPK-Induced Inhibition of Lipid Synthesis Flux in Cultured Cells
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    Chapter 24 Studying the Role of AMPK in Autophagy
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    Chapter 25 Determining AMPK Activation via the Lysosomal v-ATPase-Ragulator-AXIN/LKB1 Axis
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    Chapter 26 Manipulation and Measurement of AMPK Activity in Pancreatic Islets
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    Chapter 27 Analyzing AMPK Function in the Hypothalamus
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    Chapter 28 Using Ex Vivo Kidney Slices to Study AMPK Effects on Kidney Proteins
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    Chapter 29 A Flow Cytometry-Based Protocol to Measure Lymphocyte Viability Upon Metabolic Stress
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    Chapter 30 Methods to Evaluate AMPK Regulation of Macrophage Cholesterol Homeostasis
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    Chapter 31 Modulation of Vascular Function by AMPK: Assessment of NO Bioavailability and Surrogates of Oxidative Stress
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    Chapter 32 Measurement of Reactive Oxygen Species (ROS) and Mitochondrial ROS in AMPK Knockout Mice Blood Vessels
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    Chapter 33 Studying the Role of AMPK in Angiogenesis
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    Chapter 34 Analysis of Muscle Stem Cell Fate Through Modulation of AMPK Activity
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    Chapter 35 Evaluating the Role of Host AMPK in Leishmania Burden
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    Chapter 36 Analysis of Transgenerational Phenotypes Following Acute Starvation in AMPK-Deficient C. elegans
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    Chapter 37 Human γ2-AMPK Mutations
Attention for Chapter 13: Identifying the Heterotrimeric Complex Stoichiometry of AMPK in Skeletal Muscle by Immunoprecipitation
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Chapter title
Identifying the Heterotrimeric Complex Stoichiometry of AMPK in Skeletal Muscle by Immunoprecipitation
Chapter number 13
Book title
AMPK
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7598-3_13
Pubmed ID
Book ISBNs
978-1-4939-7597-6, 978-1-4939-7598-3
Authors

Jesper B. Birk, Jørgen F. P. Wojtaszewski

Abstract

The 5'-AMP-activated protein kinase is a complicated enzyme consisting of three different subunits, each of which is expressed as two or three isoforms. This gives the possibility of 12 different heterotrimeric complexes, which could have diverse functions within the cell. To map out which of these complexes are present and to what extent in skeletal muscle, we have used the immunoprecipitation technique and analyzed both the precipitates and the remaining supernatants for coprecipitation of complex partners. We have fine-tuned this method to give the best results on lysates from the skeletal muscle, liver, and heart muscle from mouse to man.

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The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 7 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 2 29%
Student > Bachelor 2 29%
Professor 1 14%
Student > Ph. D. Student 1 14%
Unknown 1 14%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 2 29%
Sports and Recreations 2 29%
Medicine and Dentistry 2 29%
Unknown 1 14%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 February 2018.
All research outputs
#18,589,103
of 23,025,074 outputs
Outputs from Methods in molecular biology
#7,969
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Outputs of similar age
#330,567
of 442,363 outputs
Outputs of similar age from Methods in molecular biology
#950
of 1,499 outputs
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