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Mendeley readers
Chapter title |
Determination of Adenine Nucleotide Concentrations in Cells and Tissues by High-Performance Liquid Chromatography
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Chapter number | 15 |
Book title |
AMPK
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Published in |
Methods in molecular biology, January 2018
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DOI | 10.1007/978-1-4939-7598-3_15 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7597-6, 978-1-4939-7598-3
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Authors |
Noemí García-Tardón, Bruno Guigas |
Abstract |
The serine/threonine AMP-activated protein kinase (AMPK) is a central player in the regulation of energy homeostasis, and its activity is tightly controlled, among other mechanisms, by subtle changes in cellular adenine nucleotide levels. In this chapter, we describe a step-by-step protocol for rapid, highly sensitive, reproducible, and simultaneous determination of ATP, ADP, and AMP concentrations in cell or tissue samples by reversed-phase high-performance liquid chromatography (HPLC). |
Mendeley readers
The data shown below were compiled from readership statistics for 2 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 2 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 1 | 50% |
Unknown | 1 | 50% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 1 | 50% |
Unknown | 1 | 50% |