Chapter title |
Knockdown of Human AMPK Using the CRISPR/Cas9 Genome-Editing System
|
---|---|
Chapter number | 11 |
Book title |
AMPK
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7598-3_11 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7597-6, 978-1-4939-7598-3
|
Authors |
Adrien Grenier, Pierre Sujobert, Séverine Olivier, Hélène Guermouche, Johanna Mondésir, Olivier Kosmider, Benoit Viollet, Jérôme Tamburini, Johanna Mondesir |
Abstract |
AMP-activated protein kinase (AMPK) is a critical energy sensor, regulating signaling networks involved in pathology including metabolic diseases and cancer. This increasingly recognized role of AMPK has prompted tremendous research efforts to develop new pharmacological AMPK activators. To precisely study the role of AMPK, and the specificity and activity of AMPK activators in cellular models, genetic AMPK inactivating tools are required. We report here methods for genetic inactivation of AMPK α1/α2 catalytic subunits in human cell lines by the CRISPR/Cas9 technology, a recent breakthrough technique for genome editing. |
X Demographics
Geographical breakdown
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Unknown | 3 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 3 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 19 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 6 | 32% |
Researcher | 4 | 21% |
Student > Bachelor | 3 | 16% |
Unspecified | 1 | 5% |
Unknown | 5 | 26% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 7 | 37% |
Agricultural and Biological Sciences | 2 | 11% |
Unspecified | 1 | 5% |
Nursing and Health Professions | 1 | 5% |
Psychology | 1 | 5% |
Other | 2 | 11% |
Unknown | 5 | 26% |