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Mendeley readers
Chapter title |
Analyzing AMPK Function in the Hypothalamus
|
---|---|
Chapter number | 27 |
Book title |
AMPK
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Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7598-3_27 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7597-6, 978-1-4939-7598-3
|
Authors |
Patricia Seoane-Collazo, Miguel López |
Abstract |
Hypothalamic AMPK plays a key role in the control of energy homeostasis by regulating energy intake and energy expenditure, particularly modulating brown adipose tissue (BAT) thermogenesis. The function of AMPK can be assayed by analyzing its phosphorylated protein levels in tissues, since AMPK is activated when it is phosphorylated at Thr-172. Here, we describe a method to obtain hypothalamic (nuclei-specific) protein extracts and the suitable conditions to assay AMPK phosphorylation by Western blotting. |
Mendeley readers
The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 5 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 3 | 60% |
Student > Postgraduate | 1 | 20% |
Unknown | 1 | 20% |
Readers by discipline | Count | As % |
---|---|---|
Neuroscience | 2 | 40% |
Psychology | 1 | 20% |
Unknown | 2 | 40% |