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Protein Electrophoresis

Overview of attention for book
Cover of 'Protein Electrophoresis'

Table of Contents

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    Book Overview
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    Chapter 1 How It All Began: A Personal History of Gel Electrophoresis
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    Chapter 2 Introduction to Protein Electrophoresis
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    Chapter 3 Protein Quantification Methods to Determine Protein Concentration Prior to Electrophoresis
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    Chapter 4 Protein Solubilization: Attend to the Choice of Lysis Buffer
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    Chapter 5 Native Polyacrylamide Gels
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    Chapter 6 Cationic Electrophoresis
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    Chapter 7 SARCOSYL-PAGE: A New Electrophoretic Method for the Separation and Immunological Detection of PEGylated Proteins
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    Chapter 8 Tricine-SDS-PAGE.
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    Chapter 9 Analysis of Protein Glycation Using Phenylboronate Acrylamide Gel Electrophoresis
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    Chapter 10 Protein Electrophoresis in Agarose Gels for Separating High Molecular Weight Proteins
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    Chapter 11 Increase in Local Protein Concentration by Field-Inversion Gel Electrophoresis
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    Chapter 12 A Combined Free Flow Electrophoresis and DIGE Approach to Compare Proteins in Complex Biological Samples
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    Chapter 13 Immunofixation Electrophoresis for Identification of Proteins and Specific Antibodies
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    Chapter 14 Microchip Electrophoresis, with Respect to “Profiling of Aβ Peptides in the Cerebrospinal Fluid of Patients with Alzheimer’s Disease”
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    Chapter 15 A Foodomics Approach: CE-MS for Comparative Metabolomics of Colon Cancer Cells Treated with Dietary Polyphenols
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    Chapter 16 Peptide Fractionation by SDS-Free Polyacrylamide Gel Electrophoresis for Proteomic Analysis via DF-PAGE
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    Chapter 17 Tris–Acetate Polyacrylamide Gradient Gels for the Simultaneous Electrophoretic Analysis of Proteins of Very High and Low Molecular Mass
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    Chapter 18 SDS-PAGE for 35S Immunoprecipitation and Immunoprecipitation Western Blotting
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    Chapter 19 Two-Dimensional Gel Electrophoresis: Vertical Isoelectric Focusing
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    Chapter 20 Isoelectric Focusing in Agarose Gel for Detection of Oligoclonal Bands in Cerebrospinal and Other Biological Fluids
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    Chapter 21 Native Flatbed Isoelectric Focusing for Determining Antibody Clonotype Distribution
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    Chapter 22 Two-Dimensional Gel Electrophoresis: Glass Tube-Based IEF Followed by SDS-PAGE
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    Chapter 23 One-Dimensional and Two-Dimensional Immobilized Metal Affinity Electrophoresis
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    Chapter 24 Protein Electrophoresis
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    Chapter 25 Protein Identification on Archived 2-D Gels
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    Chapter 26 Protein Electrophoresis
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    Chapter 27 Two-Dimensional Blue Native/SDS Gel Electrophoresis of Multiprotein Complexes
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    Chapter 28 Immunoelectrophoresis: A Method with Many Faces
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    Chapter 29 Cell Surface Protein Biotinylation for SDS-PAGE Analysis
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    Chapter 30 A Multichannel Gel Electrophoresis and Continuous Fraction Collection Apparatus for High-Throughput Protein Separation and Characterization
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    Chapter 31 Gel-Absorption-Based Sample Preparation Method for Shotgun Analysis of Membrane Proteome
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    Chapter 32 Interactions of Hemoglobin in Live Red Blood Cells Measured by the Electrophoresis Release Test
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    Chapter 33 Extraction of proteins from gels: a brief review.
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    Chapter 34 A New Algorithm for the Simulation of SDS 2D-PAGE Datasets
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    Chapter 35 Isolation of Proteins from Polyacrylamide Gels
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    Chapter 36 Gel Drying Methods
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    Chapter 37 Long-Term, Buffer-Less, Wet Gel Storage in Non-sealed Polyethylene Bags
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    Chapter 38 A Brief Review of Other Notable Electrophoretic Methods
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    Chapter 39 Protein Stains and Applications
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    Chapter 40 Coomassie-Brilliant Blue Staining of Polyacrylamide Gels
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    Chapter 41 Accelerated Coomassie Blue Staining and Destaining of SDS-PAGE Gels with Application of Heat
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    Chapter 42 Silver Staining Techniques of Polyacrylamide Gels
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    Chapter 43 Revisit of Imidazole–Zinc Reverse Stain for Protein Polyacrylamide Gel Electrophoresis
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    Chapter 44 Counterion Dye Staining of Proteins in One- and Two-Dimensional Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis and Tryptic Gel Digestion of Stained Protein for Mass Spectrometry
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    Chapter 45 TEMED-Enhanced Photoluminescent Imaging of Human Serum Proteins by Quantum Dots After PAGE
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    Chapter 46 Microwave-Assisted Protein Staining, Destaining, and In-Gel/In-Solution Digestion of Proteins
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    Chapter 47 Electrophoretic Separation and Detection of Metalloproteins by X-Ray Fluorescence Mapping
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    Chapter 48 Fluorescent Staining of Gels
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    Chapter 49 A Single-Step Simultaneous Protein Staining Procedure for Polyacrylamide Gels and Nitrocellulose Membranes by Alta During Western Blot Analysis
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    Chapter 50 Detection of Glycoproteins in Polyacrylamide Gels Using Pro-Q Emerald 300 Dye, a Fluorescent Periodate Schiff-Base Stain
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    Chapter 51 Spicy SDS-PAGE Gels: Curcumin/Turmeric as an Environment-Friendly Protein Stain.
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    Chapter 52 In-Gel Staining of Proteins in Native Polyacrylamide Gel Electrophoresis Using Meso-Tetrakis(4-Sulfonatophenyl) Porphyrin
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    Chapter 53 Rapid Detection of Proteins in Polyacrylamide Electrophoresis Gels with Direct Red 81 and Amido Black
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    Chapter 54 Zymographic Assay of Oxidases Using Peroxidase or Hemin Entrapped in Polyacrylamide Gel
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    Chapter 55 Coomassie Brilliant Blue Removal/Disposal from Gel Destain and Used Gel Stain in an Environment-Friendly Manner
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    Chapter 56 A Brief Review of Other Notable Protein Detection Methods on Acrylamide Gels
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    Chapter 57 Ultrasensitive Protein Detection and Imaging: Comparison of Lumitein™, ProteoSilver™, SYPRO® Ruby, and Coomassie® Brilliant Blue Gel Stains
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    Chapter 58 Common artifacts and mistakes made in electrophoresis.
Attention for Chapter 5: Native Polyacrylamide Gels
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (84th percentile)
  • High Attention Score compared to outputs of the same age and source (84th percentile)

Mentioned by

1 news outlet


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Readers on

197 Mendeley
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Chapter title
Native Polyacrylamide Gels
Chapter number 5
Book title
Protein Electrophoresis
Published in
Methods in molecular biology, January 2012
DOI 10.1007/978-1-61779-821-4_5
Pubmed ID
Book ISBNs
978-1-61779-820-7, 978-1-61779-821-4

Claudia Arndt, Stefanie Koristka, Holger Bartsch, Michael Bachmann, Arndt, Claudia, Koristka, Stefanie, Bartsch, Holger, Bachmann, Michael

Mendeley readers

The data shown below were compiled from readership statistics for 197 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 2 1%
New Zealand 1 <1%
Unknown 194 98%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 53 27%
Student > Ph. D. Student 26 13%
Student > Master 24 12%
Researcher 13 7%
Student > Doctoral Student 6 3%
Other 15 8%
Unknown 60 30%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 65 33%
Agricultural and Biological Sciences 34 17%
Chemistry 11 6%
Medicine and Dentistry 6 3%
Pharmacology, Toxicology and Pharmaceutical Science 5 3%
Other 13 7%
Unknown 63 32%

Attention Score in Context

This research output has an Altmetric Attention Score of 7. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 March 2022.
All research outputs
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Outputs from Methods in molecular biology
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Outputs of similar age
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Outputs of similar age from Methods in molecular biology
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Altmetric has tracked 23,243,271 research outputs across all sources so far. Compared to these this one has done well and is in the 80th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 13,317 research outputs from this source. They receive a mean Attention Score of 3.4. This one has done particularly well, scoring higher than 90% of its peers.
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We're also able to compare this research output to 480 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 84% of its contemporaries.