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Protein NMR Techniques

Overview of attention for book
Attention for Chapter: Residual dipolar couplings in protein structure determination.
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Chapter title
Residual dipolar couplings in protein structure determination.
Book title
Protein NMR Techniques
Published in
Methods in molecular biology, August 2004
DOI 10.1385/1-59259-809-9:089
Pubmed ID
Book ISBNs
978-1-58829-246-9, 978-1-59259-809-0
Authors

Eva de Alba, Nico Tjandra, Alba, Eva, Tjandra, Nico, Alba, Eva de

Abstract

Each magnetic nucleus behaves like a magnetic dipole able to create a local magnetic field at the position of nearby nuclei. In the presence of an external magnetic field, the local field modifies the original Larmor frequency of the affected nucleus. Such an interaction is called the dipole-dipole interaction or dipolar coupling. Its magnitude depends on, among other factors, the distance between the interacting nuclei and the angle that the internuclear vector forms with the magnetic field. Through this angular dependence it is possible to relate the position of the two interacting nuclei with respect to an arbitrary axis system of reference. Therefore, dipolar couplings can be used to obtain structural information. In liquid samples, which usually provide high-resolution nuclear magnetic resonance (NMR) spectra, the internuclear vector moves isotropically and the dipolar coupling averages to zero. In the solid state, where this vector has a fixed orientation, the dipole-dipole interactions are numerous and strong, broadening NMR signals such that structural information at high resolution cannot be obtained. An intermediate situation is achieved by partially restricting molecular tumbling of liquid samples. The alignment of a fraction of molecules in the presence of the magnetic field allows the measurement of dipolar couplings. Because they are scaled down owing to partial alignment, we refer to them as residual dipolar couplings (RDCs). The structural information obtained from RDCs has impacted enormously traditional protein structure determination based on nuclear Overhauser effect-derived distance restraints. Methods to measure RDCs and their application to protein structure determination are illustrated.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 40 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Belgium 1 3%
Brazil 1 3%
Unknown 38 95%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 11 28%
Researcher 10 25%
Student > Doctoral Student 3 8%
Student > Master 3 8%
Professor 2 5%
Other 4 10%
Unknown 7 18%
Readers by discipline Count As %
Agricultural and Biological Sciences 14 35%
Chemistry 7 18%
Biochemistry, Genetics and Molecular Biology 5 13%
Computer Science 3 8%
Medicine and Dentistry 1 3%
Other 3 8%
Unknown 7 18%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 June 2018.
All research outputs
#7,453,479
of 22,786,691 outputs
Outputs from Methods in molecular biology
#2,316
of 13,094 outputs
Outputs of similar age
#19,082
of 58,111 outputs
Outputs of similar age from Methods in molecular biology
#2
of 3 outputs
Altmetric has tracked 22,786,691 research outputs across all sources so far. This one is in the 44th percentile – i.e., 44% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,094 research outputs from this source. They receive a mean Attention Score of 3.4. This one has done well, scoring higher than 76% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 58,111 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 12th percentile – i.e., 12% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 3 others from the same source and published within six weeks on either side of this one.