| Chapter title |
Analysis of anti-interferon properties of the herpes simplex virus type I ICP0 protein.
|
|---|---|
| Book title |
Interferon Methods and Protocols
|
| Published in |
Methods in molecular medicine, July 2005
|
| DOI | 10.1385/1-59259-939-7:195 |
| Pubmed ID | |
| Book ISBNs |
978-1-58829-418-0, 978-1-59259-939-4
|
| Authors |
Karen Mossman |
| Abstract |
A defining hallmark of the type 1 interferons (IFNs) is their ability to interfere with virus replication. As such, viruses have evolved diverse mechanisms to subvert the antiviral effects of interferons. Herpes simplex virus type 1 (HSV-1) is a large deoxyribonucleic acid virus best known for its ability to cause cold sores in infected individuals. In cultured cells, HSV-1 is relatively resistant to the effects of IFNs. Plaque reduction assays were used to determine that the immediate early HSV-1 gene product ICP0 functions in part to ensure successful replication in the presence of an IFN-induced antiviral response. Northern blot analysis showed that in the absence of ICP0, HSV-1 transcript accumulation is significantly decreased. Finally, nuclear run-on assays demonstrated that ICP0 functions to overcome IFN-induced blocks to virus transcription. The methods used in these studies are described in detail in this section. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 10 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 3 | 30% |
| Researcher | 3 | 30% |
| Student > Bachelor | 3 | 30% |
| Professor > Associate Professor | 1 | 10% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 7 | 70% |
| Medicine and Dentistry | 2 | 20% |
| Unknown | 1 | 10% |