| Chapter title |
The C Type Lectins DC-SIGN and L-SIGN
|
|---|---|
| Chapter number | 4 |
| Book title |
Glycovirology Protocols
|
| Published in |
Methods in molecular biology, January 2007
|
| DOI | 10.1007/978-1-59745-393-6_4 |
| Pubmed ID | |
| Book ISBNs |
978-1-58829-590-3, 978-1-59745-393-6
|
| Authors |
Pierre-Yves Lozach, Laura Burleigh, Isabelle Staropoli, Ali Amara, Richard J. Sugrue, Lozach, Pierre-Yves, Burleigh, Laura, Staropoli, Isabelle, Amara, Ali |
| Abstract |
DC-SIGN and L-SIGN are C-type lectins that recognize carbohydrate structures present on viral glycoproteins and function as attachment factors for several enveloped viruses. DC-SIGN and L-SIGN enhance viral entry and facilitate infection of cells that express the cognate entry receptor (cis-infection). They are also able to capture viruses and transfer viral infections to other target cells (trans-infection). In this chapter, we will give an overview of protocols used to produce soluble viral glycoproteins at high levels and to study the molecular basis of viruses/DC-SIGN and L-SIGN binding and internalization. We will also describe techniques to investigate the molecular mechanisms by which DC-SIGN or L-SIGN spread viral infections. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 15 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 5 | 33% |
| Student > Master | 4 | 27% |
| Student > Doctoral Student | 1 | 7% |
| Librarian | 1 | 7% |
| Unknown | 4 | 27% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 3 | 20% |
| Medicine and Dentistry | 2 | 13% |
| Immunology and Microbiology | 2 | 13% |
| Nursing and Health Professions | 1 | 7% |
| Veterinary Science and Veterinary Medicine | 1 | 7% |
| Other | 2 | 13% |
| Unknown | 4 | 27% |