| Chapter title |
Tissue processing prior to analysis of Alzheimer's disease associated proteins and metabolites, including aβ.
|
|---|---|
| Chapter number | 33 |
| Book title |
Amyloid Proteins
|
| Published in |
Methods in molecular biology, April 2012
|
| DOI | 10.1007/978-1-61779-551-0_33 |
| Pubmed ID | |
| Book ISBNs |
978-1-61779-550-3, 978-1-61779-551-0
|
| Authors |
Schmidt SD, Nixon RA, Mathews PM, Stephen D. Schmidt, Ralph A. Nixon, Paul M. Mathews, Schmidt, Stephen D., Nixon, Ralph A., Mathews, Paul M. |
| Abstract |
Amyloid-containing tissue, whether from human patients or an animal model of a disease, is typically characterized by various biochemical and immunohistochemical techniques, many of which are described in detail in this volume. In this chapter, we describe a straightforward technique for the homogenization of tissue prior to these analyses. The technique is particularly well suited for performing a large number of different biochemical analyses on a single mouse brain hemisphere. Starting with this homogenate multiple characterizations can be done, including western blot analysis and isolation of membrane-associated proteins, both of which are described here. Additional analyses can readily be performed on the tissue homogenate, including the ELISA quantitation of Aβ in the brain of a transgenic mouse model of β-amyloid deposition. The ELISA technique is described in detail in Chapter 34. |
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