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Bacterial Chemosensing

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Cover of 'Bacterial Chemosensing'

Table of Contents

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    Book Overview
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    Chapter 1 The Diversity of Bacterial Chemosensing
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    Chapter 2 Transmembrane Signal Transduction in Bacterial Chemosensing
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    Chapter 3 Two Spatial Chemotaxis Assays: The Nutrient-Depleted Chemotaxis Assay and the Agarose-Plug-Bridge Assay
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    Chapter 4 Quantification of Bacterial Chemotaxis Responses at the Mouths of Hydrogel Capillaries
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    Chapter 5 A Static Microfluidic Device for Investigating the Chemotaxis Response to Stable, Non-linear Gradients
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    Chapter 6 Visualizing Chemoattraction of Planktonic Cells to a Biofilm
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    Chapter 7 Labeling Bacterial Flagella with Fluorescent Dyes
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    Chapter 8 All-Codon Mutagenesis for Structure-Function Studies of Chemotaxis Signaling Proteins
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    Chapter 9 Mutational Analysis of Binding Protein–Chemoreceptor Interactions
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    Chapter 10 In Vitro Assay for Measuring Receptor-Kinase Activity in the Bacillus subtilis Chemotaxis Pathway
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    Chapter 11 FRET Analysis of the Chemotaxis Pathway Response
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    Chapter 12 Monitoring Two-Component Sensor Kinases with a Chemotaxis Signal Readout
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    Chapter 13 Analyzing Protein Domain Interactions in Chemoreceptors by In Vivo PEGylation
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    Chapter 14 Tuning Chemoreceptor Signaling by Positioning Aromatic Residues at the Lipid–Aqueous Interface
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    Chapter 15 Analyzing Chemoreceptor Interactions In Vivo with the Trifunctional Cross-Linker TMEA
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    Chapter 16 Use of Cryo-EM to Study the Structure of Chemoreceptor Arrays In Vivo
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    Chapter 17 Visualizing Chemoreceptor Arrays in Bacterial Minicells by Cryo-Electron Tomography and Subtomogram Analysis
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    Chapter 18 Bacterial Chemoreceptor Imaging at High Spatiotemporal Resolution Using Photoconvertible Fluorescent Proteins
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    Chapter 19 Imaging of Single Dye-Labeled Chemotaxis Proteins in Live Bacteria Using Electroporation
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    Chapter 20 Fluorescence Anisotropy to Detect In Vivo Stimulus-Induced Changes in Chemoreceptor Packing
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    Chapter 21 Chemotaxis to Atypical Chemoattractants by Soil Bacteria
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    Chapter 22 Screening Chemoreceptor–Ligand Interactions by High-Throughput Thermal-Shift Assays
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    Chapter 23 High-Throughput Screening to Identify Chemoreceptor Ligands
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    Chapter 24 Identification of Specific Ligands for Sensory Receptors by Small-Molecule Ligand Arrays and Surface Plasmon Resonance
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    Chapter 25 Fluorescence Measurement of Kinetics of CheY Autophosphorylation with Small Molecule Phosphodonors
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    Chapter 26 Synthesis of a Stable Analog of the Phosphorylated Form of CheY: Phosphono-CheY
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    Chapter 27 Quantitative Modeling of Flagellar Motor-Mediated Adaptation
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    Chapter 28 Molecular Modeling of Chemoreceptor:Ligand Interactions
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    Chapter 29 Phylogenetic and Protein Sequence Analysis of Bacterial Chemoreceptors
Attention for Chapter 26: Synthesis of a Stable Analog of the Phosphorylated Form of CheY: Phosphono-CheY
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Chapter title
Synthesis of a Stable Analog of the Phosphorylated Form of CheY: Phosphono-CheY
Chapter number 26
Book title
Bacterial Chemosensing
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7577-8_26
Pubmed ID
Book ISBNs
978-1-4939-7576-1, 978-1-4939-7577-8
Authors

Daniel B. Lookadoo, Matthew S. Beyersdorf, Christopher J. Halkides

Abstract

CheY is a response regulator of bacterial chemotaxis that is activated by phosphorylation of a conserved aspartate residue. However, studies of CheY-phosphate have proven challenging due to rapid hydrolysis of the aspartyl-phosphate in vitro. To combat this issue, we have designed a stable analog suitable for structural and functional studies. Herein, we describe a method for the chemical modification of Thermotoga maritima CheY to produce a phospho-analog designated as phosphono-CheY. Our modification produces a stable analog in the constitutively active form that enables the study of signal transfer to the downstream target.

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Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 February 2018.
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#18,587,406
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#950
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