Chapter title |
Creation of transgenic lines using microparticle bombardment methods.
|
---|---|
Book title |
C. elegans
|
Published in |
Methods in molecular biology, September 2006
|
DOI | 10.1385/1-59745-151-7:93 |
Pubmed ID | |
Book ISBNs |
978-1-58829-597-2, 978-1-59745-151-2
|
Authors |
Praitis V, Vida Praitis, Praitis, Vida |
Abstract |
Introduction of exogenous DNA into Caenorhabditis elegans is important for examining the expression of altered or reporter gene constructs, rescuing mutant genes, and studying gene function in vivo. Until recently, germ-line injection was the most commonly used method for transforming C. elegans strains. This chapter describes four different microparticle bombardment methods used to transform C. elegans with exogenous DNA. We include a discussion of the advantages and disadvantages of using microparticle bombardment for transformation, list cotransformation markers that have been used successfully in microparticle bombardment experiments, and discuss transformation efficiency. |
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