Chapter title |
Selective Targeting of CD133-Expressing Glioblastoma Stem Cells Using Lentiviral Vectors
|
---|---|
Chapter number | 7 |
Book title |
Glioblastoma
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7659-1_7 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7658-4, 978-1-4939-7659-1
|
Authors |
N. Sumru Bayin, Dimitris G. Placantonakis |
Abstract |
Several lines of evidence suggest a cellular hierarchy in glioblastoma (GBM). In this hierarchy, GBM stem-like cells (GSCs) play critical roles in tumor progression and recurrence, by virtue of their robust tumor-propagating potential and resistance to conventional chemoradiotherapy. Therefore, targeting GSCs holds significant therapeutic promise. Expression of CD133 (PROM1), a cell surface glycoprotein, has been associated with the GSC phenotype and used as a GSC marker. Here, we describe a protocol that allows the selective lentiviral transduction of CD133-expressing GBM cells. This selectivity is conferred by pseudotyping the lentiviral envelope with a single-chain antibody against an extracellular epitope on CD133. We previously demonstrated the efficacy and specificity of this lentiviral vector using patient-derived GBM cultures. This chapter outlines the preparation of the vector and the transduction of human GBM cells. |
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