Chapter title |
Orthotopic Patient-Derived Glioblastoma Xenografts in Mice
|
---|---|
Chapter number | 14 |
Book title |
Glioblastoma
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7659-1_14 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7658-4, 978-1-4939-7659-1
|
Authors |
Zhongye Xu, Michael Kader, Rajeev Sen, Dimitris G. Placantonakis |
Abstract |
Patient-derived xenografts (PDX) provide in vivo glioblastoma (GBM) models that recapitulate actual tumors. Orthotopic tumor xenografts within the mouse brain are obtained by injection of GBM stem-like cells derived from fresh surgical specimens. These xenografts reproduce GBM's histologic complexity and hallmark biological behaviors, such as brain invasion, angiogenesis, and resistance to therapy. This method has become essential for analyzing mechanisms of tumorigenesis and testing the therapeutic effect of candidate agents in the preclinical setting. Here, we describe a protocol for establishing orthotopic tumor xenografts in the mouse brain with human GBM cells. |
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Other | 4 | 11% |
Student > Master | 4 | 11% |
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Student > Bachelor | 2 | 6% |
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Chemistry | 2 | 6% |
Other | 4 | 11% |
Unknown | 11 | 31% |