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Co-culture of human fibroblasts and Borrelia burgdorferi enhances collagen and growth factor mRNA

Overview of attention for article published in Archives of Dermatological Research, December 2017
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • Among the highest-scoring outputs from this source (#31 of 1,242)
  • High Attention Score compared to outputs of the same age (88th percentile)
  • Above-average Attention Score compared to outputs of the same age and source (60th percentile)

Mentioned by

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20 tweeters
facebook
3 Facebook pages
video
1 video uploader

Citations

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6 Dimensions

Readers on

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26 Mendeley
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Title
Co-culture of human fibroblasts and Borrelia burgdorferi enhances collagen and growth factor mRNA
Published in
Archives of Dermatological Research, December 2017
DOI 10.1007/s00403-017-1797-1
Pubmed ID
Authors

Elisabeth Aberer, Milana Surtov-Pudar, Daniel Wilfinger, Alexander Deutsch, Gerd Leitinger, Helmut Schaider

Abstract

Skin fibrosis has been reported in Borrelia burgdorferi infection in Europe, but has been questioned by several authors. The objective of the present study was to examine the interaction of skin fibroblasts with B. burgdorferi sensu stricto B31 (BB) and B. afzelii (BA) in vitro by electron microscopy. We also determined the expression of collagen type I, TGF-β, FGF-1, calreticulin (CALR), decorin (DCN), and PDGF-α at the mRNA level in Borrelia/fibroblast co-cultures. Intact Borrelia attach to and transmigrate fibroblasts, and undergo cystic transformation outside the fibroblasts. Fibroblasts preserve their vitality and express a prominent granular endoplasmic reticulum, suggesting activated protein synthesis. On two different semi-quantitative real-time PCR assays, BB- and BA/fibroblast co-cultures showed a significant induction of type I collagen mRNA after 2 days compared to fibroblasts (fourfold for BA and 1.8-fold for BB; p < 0.02). In addition, there was a significant upregulation of mRNA expression of TGF-β, CALR, PDGF-α, and DCN in BA and BB co-cultures compared to control fibroblasts in monolayer cultures after 2 days (p < 0.01). The BA/fibroblast co-culture induced a considerably greater upregulation of collagen and growth factor mRNA compared to BB/fibroblast co-culture. In contrast, a significant down-regulation of FGF-1 (20-fold for BA and 4.5-fold for BB) mRNA expression was detected in co-cultures compared to controls (p < 0.01). The results of the study support the hypothesis that BB sensu lato, and BA in particular, enhances collagen mRNA expression and can stimulate growth factors responsible for increased collagen production.

Twitter Demographics

The data shown below were collected from the profiles of 20 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 26 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 26 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 8 31%
Librarian 7 27%
Other 3 12%
Student > Ph. D. Student 3 12%
Student > Bachelor 2 8%
Other 2 8%
Unknown 1 4%
Readers by discipline Count As %
Veterinary Science and Veterinary Medicine 6 23%
Immunology and Microbiology 6 23%
Medicine and Dentistry 5 19%
Agricultural and Biological Sciences 5 19%
Biochemistry, Genetics and Molecular Biology 3 12%
Other 0 0%
Unknown 1 4%

Attention Score in Context

This research output has an Altmetric Attention Score of 17. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 February 2022.
All research outputs
#1,696,813
of 21,063,002 outputs
Outputs from Archives of Dermatological Research
#31
of 1,242 outputs
Outputs of similar age
#44,484
of 395,948 outputs
Outputs of similar age from Archives of Dermatological Research
#2
of 5 outputs
Altmetric has tracked 21,063,002 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 91st percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,242 research outputs from this source. They receive a mean Attention Score of 4.3. This one has done particularly well, scoring higher than 97% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 395,948 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 88% of its contemporaries.
We're also able to compare this research output to 5 others from the same source and published within six weeks on either side of this one. This one has scored higher than 3 of them.