Chapter title |
Introduction of Genetic Material in Ralstonia solanacearum Through Natural Transformation and Conjugation
|
---|---|
Chapter number | 16 |
Book title |
Host-Pathogen Interactions
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7604-1_16 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7603-4, 978-1-4939-7604-1
|
Authors |
Anthony Perrier, Patrick Barberis, Stéphane Genin |
Abstract |
Ralstonia solanacearum is a soil-borne plant pathogen, responsible of the bacterial wilt disease. Its unusual wide host range (more than 250 plant species), aggressiveness, and broad geographic distribution have made of this bacterium the main plant pathogenic model in the beta-Proteobacteria class. Many R. solanacearum strains have the ability to internalize exogenous DNA through natural transformation. This property is widely used in reverse genetics studies to create mutants or reporter gene constructs, in the aim to study the molecular bases of pathogenesis of this bacterium. In this chapter, we describe three in vitro methods (natural transformation, electrotransformation, and conjugation) commonly used to produce recombinant R. solanacearum cells after introduction of exogenous DNA. |
X Demographics
As of 1 July 2024, you may notice a temporary increase in the numbers of X profiles with Unknown location. Click here to learn more.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 16 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 6 | 38% |
Student > Doctoral Student | 2 | 13% |
Researcher | 1 | 6% |
Student > Master | 1 | 6% |
Unknown | 6 | 38% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 8 | 50% |
Veterinary Science and Veterinary Medicine | 1 | 6% |
Biochemistry, Genetics and Molecular Biology | 1 | 6% |
Immunology and Microbiology | 1 | 6% |
Unknown | 5 | 31% |