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Peptide Libraries

Overview of attention for book
Cover of 'Peptide Libraries'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Design, Synthesis, and Application of OB2C Combinatorial Peptide and Peptidomimetic Libraries.
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    Chapter 2 Synthesis of Macrocyclic Organo-peptide Hybrids from Ribosomal Polypeptide Precursors via CuAAC-/Hydrazide-Mediated Cyclization.
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    Chapter 3 Synthesis and screening of one-bead-one-compound cyclic Peptide libraries.
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    Chapter 4 Creating Site-Specific Isopeptide Linkages Between Proteins with the Traceless Staudinger Ligation
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    Chapter 5 Macrocyclic Templates for Library Synthesis of Peptido-Conjugates
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    Chapter 6 Synthesis and alkylation of aza-gly-pro building blocks of peptidomimetic libraries for developing prostaglandin f2α receptor modulators as therapeutics to inhibit preterm labor.
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    Chapter 7 A Fragment-Based Selection Approach for the Discovery of Peptide Macrocycles Targeting Protein Kinases
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    Chapter 8 Peptide and Peptide Library Cyclization via Bromomethylbenzene Derivatives.
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    Chapter 9 High order unconditionally stable difference schemes for the Riesz space-fractional telegraph equation
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    Chapter 10 De Novo Discovery of Bioactive Cyclic Peptides Using Bacterial Display and Flow Cytometry
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    Chapter 11 Chemical Posttranslational Modification of Phage-Displayed Peptides
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    Chapter 12 Mapping protein-protein interactions with phage-displayed combinatorial Peptide libraries and alanine scanning.
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    Chapter 13 Identifying Reactive Peptides from Phage-Displayed Libraries
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    Chapter 14 The best and the brightest: exploiting tryptophan-sensitized tb(3+) luminescence to engineer lanthanide-binding tags.
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    Chapter 15 Synthesis and Cell-Based Screening of One-Bead-One-Compound Peptide Libraries
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    Chapter 16 Screening Peptide Array Library for the Identification of Cancer Cell-Binding Peptides
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    Chapter 17 Next-generation sequencing of phage-displayed Peptide libraries.
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    Chapter 18 Maleimide-Based Method for Elaboration of Cysteine-Containing Peptide Phage Libraries
Attention for Chapter 17: Next-generation sequencing of phage-displayed Peptide libraries.
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Chapter title
Next-generation sequencing of phage-displayed Peptide libraries.
Chapter number 17
Book title
Peptide Libraries
Published in
Methods in molecular biology, January 2015
DOI 10.1007/978-1-4939-2020-4_17
Pubmed ID
Book ISBNs
978-1-4939-2019-8, 978-1-4939-2020-4
Authors

Wadim L Matochko, Ratmir Derda, Wadim L. Matochko, Matochko, Wadim L., Derda, Ratmir

Abstract

Genetically encoded peptide libraries enabled the discovery of ligands for clinically relevant targets and functional materials. Next-generation sequencing (NGS) of these libraries improved the selection of ligands by detecting low abundant clones and quantifying changes in copy numbers of clones without many rounds of selection. Although NGS platforms have been widely used in genome assembly, quantification of gene expression (RNA-seq), and metagenomic analyses, few examples in the literature describe sequencing phage libraries. This chapter aims to provide a detailed method for sequencing a Ph.D.-7 phage display library by Ion Torrent. The main techniques covered in this chapter include (1) preparation of a phage library for sequencing, (2) sequencing, and (3) analysis of the sequencing data by a custom Matlab script.

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The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 61 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 61 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 14 23%
Researcher 13 21%
Student > Master 8 13%
Student > Bachelor 8 13%
Other 3 5%
Other 3 5%
Unknown 12 20%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 20 33%
Agricultural and Biological Sciences 8 13%
Chemistry 5 8%
Pharmacology, Toxicology and Pharmaceutical Science 3 5%
Engineering 2 3%
Other 7 11%
Unknown 16 26%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 September 2015.
All research outputs
#14,392,043
of 23,498,099 outputs
Outputs from Methods in molecular biology
#4,089
of 13,368 outputs
Outputs of similar age
#185,311
of 356,521 outputs
Outputs of similar age from Methods in molecular biology
#260
of 1,005 outputs
Altmetric has tracked 23,498,099 research outputs across all sources so far. This one is in the 37th percentile – i.e., 37% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,368 research outputs from this source. They receive a mean Attention Score of 3.4. This one has gotten more attention than average, scoring higher than 67% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 356,521 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 46th percentile – i.e., 46% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 1,005 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 72% of its contemporaries.