Chapter title |
Cortical Cell Length Analysis During Gravitropic Root Growth
|
---|---|
Chapter number | 14 |
Book title |
Root Development
|
Published in |
Methods in molecular biology, March 2018
|
DOI | 10.1007/978-1-4939-7747-5_14 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7746-8, 978-1-4939-7747-5
|
Authors |
Maria Schöller, Jürgen Kleine-Vehn, Elena Feraru |
Abstract |
The typical parameter used to evaluate the root growth response to gravity is the degree of root bending in time. This employs the quantification of the root tip angle toward gravity and, hence, does not directly assess the actual differential growth process. Here, we describe the cortical cell length as a parameter to quantify cell elongation during the gravitropic response, using median longitudinal confocal sections. This analysis depicts that root organ bending is a consequence of differential cellular elongation on the upper versus lower side of the gravistimulated root. Moreover, we introduce here a simple mounting setup that is suitable to gravistimulate and subsequently image seedlings on upright microscopes. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 6 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 3 | 50% |
Professor > Associate Professor | 1 | 17% |
Researcher | 1 | 17% |
Unknown | 1 | 17% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 3 | 50% |
Biochemistry, Genetics and Molecular Biology | 2 | 33% |
Unknown | 1 | 17% |