Chapter title |
Growth Rate Normalization Method to Assess Gravitropic Root Growth
|
---|---|
Chapter number | 15 |
Book title |
Root Development
|
Published in |
Methods in molecular biology, March 2018
|
DOI | 10.1007/978-1-4939-7747-5_15 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7746-8, 978-1-4939-7747-5
|
Authors |
Maria Schöller, Elizabeth Sarkel, Jürgen Kleine-Vehn, Elena Feraru |
Abstract |
Time-lapse imaging of roots is highly suitable for depicting gravitropic growth behaviors. However, roots may show faster or slower bending kinetics when compared to control as a result of differences in overall root growth. Accordingly, conditions that cause differential organ growth require growth rate normalization to compare gravitropic curvature. Here, we describe a simple normalization method for gravitropic root growth evaluation. We exemplify this method by exposing seedlings to distinct environmental conditions or disturbing the cellular auxin contents. This data shows that the method is suitable to discriminate between gravitropic and overall organ growth defects. |
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