Chapter title |
Monitoring Transcriptomic Changes in Soil-Grown Roots and Shoots of Arabidopsis thaliana Subjected to a Progressive Drought Stress
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Chapter number | 17 |
Book title |
Root Development
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Published in |
Methods in molecular biology, March 2018
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DOI | 10.1007/978-1-4939-7747-5_17 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7746-8, 978-1-4939-7747-5
|
Authors |
Khurram Bashir, Sultana Rasheed, Akihiro Matsui, Kei Iida, Maho Tanaka, Motoaki Seki, Bashir, Khurram, Rasheed, Sultana, Matsui, Akihiro, Iida, Kei, Tanaka, Maho, Seki, Motoaki |
Abstract |
Numerous experiments have been performed in Arabidopsis to monitor changes in gene expression that occur in response to a variety of abiotic and biotic stresses, different growth conditions, and at various developmental stages. In addition, gene expression patterns have also been characterized among wild-type and mutant genotypes. Despite these numerous reports, transcriptional changes occurring in roots of soil-grown plants subjected to a progressive drought stress have remained undocumented. To fill this gap, we established a system that allows one to establish water-deficit conditions and to collect root and shoot samples with minimal damage to the root system. Arabidopsis plants are grown in a ceramic-based granular soil and subjected to progressive drought stress by withholding water. Root and shoot samples were collected separately, RNA was purified, and a microarray analysis of drought-stressed roots and shoots was performed at 0, 1, 3, 5, 7, and 9 days after the onset of drought stress treatment. Here, we describe the detailed protocol used to analyze the transcriptomic changes occurring in roots and shoots of soil-grown Arabidopsis subjected to a progressive drought stress. |
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