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Root Development

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Cover of 'Root Development'

Table of Contents

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    Book Overview
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    Chapter 1 Measuring Plant Root Traits Under Controlled and Field Conditions: Step-by-Step Procedures
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    Chapter 2 Phenotyping Crop Root Crowns: General Guidance and Specific Protocols for Maize, Wheat, and Soybean
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    Chapter 3 Developmental Analysis of Arabidopsis Root Meristem
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    Chapter 4 Genetic and Phenotypic Analysis of Lateral Root Development in Arabidopsis thaliana
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    Chapter 5 Adapting the Lateral Root-Inducible System to Medicago truncatula
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    Chapter 6 Characterization of Root Epidermal Cell Patterning and Differentiation in Arabidopsis
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    Chapter 7 In Vitro Assay for Induction of Adventitious Rooting on Intact Arabidopsis Hypocotyls
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    Chapter 8 Root Gravitropism: Quantification, Challenges, and Solutions
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    Chapter 9 Calcium Ion Dynamics in Roots: Imaging and Analysis
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    Chapter 10 Optimized Whole-Mount In Situ Immunolocalization for Arabidopsis thaliana Root Meristems and Lateral Root Primordia
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    Chapter 11 Light Sheet Fluorescence Microscopy Optimized for Long-Term Imaging of Arabidopsis Root Development
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    Chapter 12 Histological Profiling Over Time to Optimize Root Cell Type-Specific Reporter Lines for Cell Sorting
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    Chapter 13 Long-Term In Vivo Imaging of Luciferase-Based Reporter Gene Expression in Arabidopsis Roots
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    Chapter 14 Cortical Cell Length Analysis During Gravitropic Root Growth
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    Chapter 15 Growth Rate Normalization Method to Assess Gravitropic Root Growth
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    Chapter 16 Immunoprecipitation of Membrane Proteins from Arabidopsis thaliana Root Tissue
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    Chapter 17 Monitoring Transcriptomic Changes in Soil-Grown Roots and Shoots of Arabidopsis thaliana Subjected to a Progressive Drought Stress
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    Chapter 18 Chromatin Immunoprecipitation Sequencing (ChIP-Seq) for Transcription Factors and Chromatin Factors in Arabidopsis thaliana Roots: From Material Collection to Data Analysis
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    Chapter 19 μChIP-Seq for Genome-Wide Mapping of In Vivo TF-DNA Interactions in Arabidopsis Root Protoplasts
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    Chapter 20 Proteome Analysis of Arabidopsis Roots
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    Chapter 21 Step-by-Step Construction of Gene Co-expression Networks from High-Throughput Arabidopsis RNA Sequencing Data
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    Chapter 22 GWA-Portal: Genome-Wide Association Studies Made Easy
Attention for Chapter 9: Calcium Ion Dynamics in Roots: Imaging and Analysis
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Chapter title
Calcium Ion Dynamics in Roots: Imaging and Analysis
Chapter number 9
Book title
Root Development
Published in
Methods in molecular biology, March 2018
DOI 10.1007/978-1-4939-7747-5_9
Pubmed ID
Book ISBNs
978-1-4939-7746-8, 978-1-4939-7747-5
Authors

Ellie Himschoot, Melanie Krebs, Alex Costa, Tom Beeckman, Steffen Vanneste

Abstract

Calcium sensors are indispensable tools to study the role of Ca2+ and visualize Ca2+ dynamics during biological processes. Over the past years, the field of Ca2+ imaging has strongly expanded by the development of a wide palette of sensors and optimization of sample handling. Here, we provide guidelines for imaging of the Ca2+ sensor R-GECO1 in Arabidopsis thaliana roots which can be interpolated to other intensiometric Ca2+ sensors. Furthermore, we demonstrate a procedure for image analysis of the acquired time-lapse recordings.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 11 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 11 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 4 36%
Student > Ph. D. Student 2 18%
Student > Master 2 18%
Other 1 9%
Professor > Associate Professor 1 9%
Other 0 0%
Unknown 1 9%
Readers by discipline Count As %
Agricultural and Biological Sciences 5 45%
Biochemistry, Genetics and Molecular Biology 4 36%
Physics and Astronomy 1 9%
Unknown 1 9%