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Nuclear Bodies and Noncoding RNAs

Overview of attention for book
Cover of 'Nuclear Bodies and Noncoding RNAs'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Visualization of lncRNA by Single-Molecule Fluorescence In Situ Hybridization
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    Chapter 2 Super-Resolution Imaging of Nuclear Bodies by STED Microscopy.
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    Chapter 3 High-Resolution 3D DNA FISH Using Plasmid Probes and Computational Correction of Optical Aberrations to Study Chromatin Structure at the Sub-megabase Scale.
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    Chapter 4 Time-Lapse Imaging of Nuclear Bodies
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    Chapter 5 Visualization of Nucleic Acids with Synthetic Exciton-Controlled Fluorescent Oligonucleotide Probes
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    Chapter 6 Live CLEM Imaging to Analyze Nuclear Structures at High Resolution
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    Chapter 7 Ultrastructural Analysis of Nuclear Bodies Using Electron Microscopy
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    Chapter 8 Analyses of Nuclear Proteins and Nucleic Acid Structures Using Atomic Force Microscopy.
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    Chapter 9 Genome-Wide Co-Localization Screening of Nuclear Body Components Using a Fluorescently Tagged FLJ cDNA Clone Library.
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    Chapter 10 Purification of Specific Chromatin Regions Using Oligonucleotides: Capture Hybridization Analysis of RNA Targets (CHART).
  12. Altmetric Badge
    Chapter 11 RNA Antisense Purification (RAP) for Mapping RNA Interactions with Chromatin.
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    Chapter 12 In Situ Dissection of RNA Functional Subunits by Domain-Specific Chromatin Isolation by RNA Purification (dChIRP).
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    Chapter 13 Extracting, Enriching, and Identifying Nuclear Body Sub-Complexes Using Label-Based Quantitative Mass Spectrometry.
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    Chapter 14 Studying RNA-Binding Protein Interactions with Target mRNAs in Eukaryotic Cells: Native Ribonucleoprotein Immunoprecipitation (RIP) Assays
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    Chapter 15 Cross-Linking and Immunoprecipitation of Nuclear RNA-Binding Proteins
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    Chapter 16 Purification of Noncoding RNA and Bound Proteins Using FLAG Peptide-Conjugated Antisense-Oligonucleotides.
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    Chapter 17 MMCT-Mediated Chromosome Engineering Technique Applicable to Functional Analysis of lncRNA and Nuclear Dynamics
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    Chapter 18 Reconstitution of Nucleocytoplasmic Transport Using Digitonin-Permeabilized Cells
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    Chapter 19 Genome-Wide Analysis of Long Noncoding RNA Turnover.
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    Chapter 20 Knockdown of Nuclear-Retained Long Noncoding RNAs Using Modified DNA Antisense Oligonucleotides.
  22. Altmetric Badge
    Chapter 21 siRNA Screening of Nuclear Proteins
Attention for Chapter 10: Purification of Specific Chromatin Regions Using Oligonucleotides: Capture Hybridization Analysis of RNA Targets (CHART).
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  • Good Attention Score compared to outputs of the same age and source (69th percentile)

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Chapter title
Purification of Specific Chromatin Regions Using Oligonucleotides: Capture Hybridization Analysis of RNA Targets (CHART).
Chapter number 10
Book title
Nuclear Bodies and Noncoding RNAs
Published in
Methods in molecular biology, January 2015
DOI 10.1007/978-1-4939-2253-6_10
Pubmed ID
25555581
Book ISBNs
978-1-4939-2252-9, 978-1-4939-2253-6
Authors

Christopher P Davis, Jason A West, Christopher P. Davis, Jason A. West

Abstract

Identification of genomic binding sites and proteins associated with noncoding RNAs will lead to more complete mechanistic characterization of the regulatory activities of noncoding RNAs. Capture hybridization analysis of RNA targets (CHART) is a powerful technique wherein specific RNA molecules are isolated from cross-linked nuclear extracts using complementary, biotinylated capture oligonucleotides, allowing subsequent identification of genomic DNA and proteins cross-linked to the RNA of interest. Here, we describe the procedure for CHART and list strategies to optimize nuclear extract preparation, capture oligonucleotide design, and isolation of nucleic acids and proteins enriched through CHART.

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X Demographics

X Demographics

The data shown below were collected from the profiles of 4 X users who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 23 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 23 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 7 30%
Researcher 5 22%
Professor 3 13%
Professor > Associate Professor 2 9%
Student > Bachelor 1 4%
Other 3 13%
Unknown 2 9%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 10 43%
Agricultural and Biological Sciences 10 43%
Neuroscience 1 4%
Unknown 2 9%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 September 2015.
All research outputs
#14,718,998
of 23,577,654 outputs
Outputs from Methods in molecular biology
#4,374
of 13,410 outputs
Outputs of similar age
#190,790
of 356,631 outputs
Outputs of similar age from Methods in molecular biology
#280
of 1,000 outputs
Altmetric has tracked 23,577,654 research outputs across all sources so far. This one is in the 35th percentile – i.e., 35% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,410 research outputs from this source. They receive a mean Attention Score of 3.4. This one has gotten more attention than average, scoring higher than 63% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 356,631 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 43rd percentile – i.e., 43% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 1,000 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 69% of its contemporaries.

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