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Next Generation Sequencing

Overview of attention for book
Cover of 'Next Generation Sequencing'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 An Integrated Polysome Profiling and Ribosome Profiling Method to Investigate In Vivo Translatome
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    Chapter 2 Measuring Nascent Transcripts by Nascent-seq
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    Chapter 3 Genome-Wide Copy Number Alteration Detection in Preimplantation Genetic Diagnosis
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    Chapter 4 Multiplexed Targeted Sequencing for Oxford Nanopore MinION: A Detailed Library Preparation Procedure
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    Chapter 5 Hi-Plex for Simple, Accurate, and Cost-Effective Amplicon-based Targeted DNA Sequencing
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    Chapter 6 ClickSeq: Replacing Fragmentation and Enzymatic Ligation with Click-Chemistry to Prevent Sequence Chimeras
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    Chapter 7 Genome-Wide Analysis of DNA Methylation in Single Cells Using a Post-bisulfite Adapter Tagging Approach
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    Chapter 8 Sequencing of Genomes from Environmental Single Cells
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    Chapter 9 SNP Discovery from Single and Multiplex Genome Assemblies of Non-model Organisms
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    Chapter 10 CleanTag Adapters Improve Small RNA Next-Generation Sequencing Library Preparation by Reducing Adapter Dimers
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    Chapter 11 Sampling, Extraction, and High-Throughput Sequencing Methods for Environmental Microbial and Viral Communities
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    Chapter 12 A Bloody Primer: Analysis of RNA-Seq from Tissue Admixtures
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    Chapter 13 Next-Generation Sequencing of Genome-Wide CRISPR Screens
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    Chapter 14 Gene Profiling and T Cell Receptor Sequencing from Antigen-Specific CD4 T Cells
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    Chapter 15 Investigate Global Chromosomal Interaction by Hi-C in Human Naive CD4 T Cells
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    Chapter 16 Primer Extension, Capture, and On-Bead cDNA Ligation: An Efficient RNAseq Library Prep Method for Determining Reverse Transcription Termination Sites
Attention for Chapter 13: Next-Generation Sequencing of Genome-Wide CRISPR Screens
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • Good Attention Score compared to outputs of the same age (76th percentile)
  • High Attention Score compared to outputs of the same age and source (91st percentile)

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8 X users
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1 Wikipedia page

Readers on

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190 Mendeley
Summary X Wikipedia
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Chapter title
Next-Generation Sequencing of Genome-Wide CRISPR Screens
Chapter number 13
Book title
Next Generation Sequencing
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7514-3_13
Pubmed ID
29224076
Book ISBNs
978-1-4939-7512-9, 978-1-4939-7514-3
Authors

Edwin H. Yau, Tariq M. Rana, Yau, Edwin H., Rana, Tariq M.

Abstract

Genome-wide functional genomic screens utilizing the clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 system have proven to be a powerful tool for systematic genomic perturbation in mammalian cells and provide an alternative to previous screens utilizing RNA interference technology. The wide availability of these libraries through public plasmid repositories as well as the decreasing cost and speed in quantifying these screens using high-throughput next-generation sequencing (NGS) allows for the adoption of the technology in a variety of laboratories interested in diverse biologic questions. Here, we describe the protocol to generate next-generation sequencing libraries from genome-wide CRISPR genomic screens.

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X Demographics

X Demographics

The data shown below were collected from the profiles of 8 X users who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 190 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 190 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 36 19%
Researcher 34 18%
Student > Bachelor 24 13%
Student > Master 20 11%
Other 9 5%
Other 14 7%
Unknown 53 28%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 75 39%
Agricultural and Biological Sciences 24 13%
Medicine and Dentistry 10 5%
Immunology and Microbiology 8 4%
Chemistry 4 2%
Other 9 5%
Unknown 60 32%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 8. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 February 2020.
All research outputs
#4,805,073
of 23,577,654 outputs
Outputs from Methods in molecular biology
#1,407
of 13,410 outputs
Outputs of similar age
#103,167
of 445,160 outputs
Outputs of similar age from Methods in molecular biology
#119
of 1,482 outputs
Altmetric has tracked 23,577,654 research outputs across all sources so far. Compared to these this one has done well and is in the 79th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 13,410 research outputs from this source. They receive a mean Attention Score of 3.4. This one has done well, scoring higher than 89% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 445,160 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 76% of its contemporaries.
We're also able to compare this research output to 1,482 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 91% of its contemporaries.

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