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Nuclear Reprogramming

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Cover of 'Nuclear Reprogramming'

Table of Contents

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    Book Overview
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    Chapter 1 Nuclear Transfer in the Mouse
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    Chapter 2 Nuclear Transfer in Rabbit
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    Chapter 3 Nuclear Transfer in Ruminants
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    Chapter 4 Nuclear Transfer and Transgenesis in the Pig
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    Chapter 5 Nuclear Reprogramming
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    Chapter 6 Analysis of nuclear reprogramming following nuclear transfer to Xenopus oocyte.
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    Chapter 7 Assessing the Quality of Donor Cells: Karyotyping Methods
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    Chapter 8 Treatment of Donor Cell/Embryo with Different Approaches to Improve Development After Nuclear Transfer
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    Chapter 9 Fluorescent immunodetection of epigenetic modifications on preimplantation mouse embryos.
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    Chapter 10 Visualization of epigenetic modifications in preimplantation embryos.
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    Chapter 11 Live Embryo Imaging to Follow Cell Cycle and Chromosomes Stability After Nuclear Transfer
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    Chapter 12 Analysis of nucleolar morphology and protein localization as an indicator of nuclear reprogramming.
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    Chapter 13 Assessment of Cell Lineages and Cell Death in Blastocysts by Immunostaining
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    Chapter 14 Gene Expression Analysis in Early Embryos Through Reverse Transcription Quantitative PCR (RT-qPCR)
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    Chapter 15 Studying Bovine Early Embryo Transcriptome by Microarray
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    Chapter 16 Methylation of Specific Regions: Bisulfite-Sequencing at the Single Oocyte or 2-Cell Embryo Level
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    Chapter 17 Micro Chromatin Immunoprecipitation (μChIP) from Early Mammalian Embryos.
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    Chapter 18 Assessing reprogramming by chimera formation and tetraploid complementation.
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    Chapter 19 Whole-Mount In Situ Hybridization to Assess Advancement of Development and Embryo Morphology
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    Chapter 20 Genome-Wide Analysis of Methylation in Bovine Clones by Methylated DNA Immunoprecipitation (MeDIP)
Attention for Chapter 20: Genome-Wide Analysis of Methylation in Bovine Clones by Methylated DNA Immunoprecipitation (MeDIP)
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Chapter title
Genome-Wide Analysis of Methylation in Bovine Clones by Methylated DNA Immunoprecipitation (MeDIP)
Chapter number 20
Book title
Nuclear Reprogramming
Published in
Methods in molecular biology, January 2015
DOI 10.1007/978-1-4939-1594-1_20
Pubmed ID
Book ISBNs
978-1-4939-1593-4, 978-1-4939-1594-1
Authors

Hélène Kiefer, Kiefer, Hélène

Editors

Nathalie Beaujean, Hélène Jammes, Alice Jouneau

Abstract

Methylated DNA immunoprecipitation (MeDIP), when coupled to high-throughput sequencing or microarray hybridization, allows for the identification of methylated loci at a genome-wide scale. Genomic regions affected by incomplete reprogramming after nuclear transfer can potentially be delineated by comparing the MeDIP profiles of bovine clones and non-clones. This chapter presents a MeDIP protocol largely inspired from Mohn and colleagues (Mohn et al., Methods Mol Biol 507:55-64, 2009), with PCR primers specific for cattle, and when possible, overviews of experimental designs adapted to the comparison between clones and non-clones.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 4 100%

Demographic breakdown

Readers by professional status Count As %
Professor > Associate Professor 1 25%
Researcher 1 25%
Student > Doctoral Student 1 25%
Unknown 1 25%
Readers by discipline Count As %
Agricultural and Biological Sciences 2 50%
Nursing and Health Professions 1 25%
Unknown 1 25%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 December 2014.
All research outputs
#20,246,428
of 22,774,233 outputs
Outputs from Methods in molecular biology
#9,866
of 13,091 outputs
Outputs of similar age
#295,648
of 352,928 outputs
Outputs of similar age from Methods in molecular biology
#635
of 996 outputs
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So far Altmetric has tracked 13,091 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 996 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.