| Chapter title |
Optimized Specific Isolation of Placenta-Derived Exosomes from Maternal Circulation
|
|---|---|
| Chapter number | 10 |
| Book title |
Preeclampsia
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7498-6_10 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7497-9, 978-1-4939-7498-6
|
| Authors |
Andrew Lai, Omar Elfeky, Gregory E. Rice, Carlos Salomon, Lai, Andrew, Elfeky, Omar, Rice, Gregory E., Salomon, Carlos |
| Abstract |
Exosomes are small (~100 nm) vesicles that carry a wide range of molecules including proteins, RNAs, and DNA. Exosomes are secreted from a wide range of cells including placental cells. Interestingly, exosomes secreted from placental cells have been identified in maternal circulation as early as in 6 weeks of gestation, and their concentration increases with the gestational age. While there is growing interest in elucidating the role of exosomes during normal and complicated pregnancies (such as preeclampsia), progress in the field has been delayed because of the inability to isolate placental exosomes from maternal circulation. Therefore, here we describe a workflow to isolate placental exosomes from maternal circulation. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 50% |
| Scientists | 1 | 50% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 39 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 12 | 31% |
| Student > Bachelor | 4 | 10% |
| Student > Doctoral Student | 3 | 8% |
| Researcher | 3 | 8% |
| Student > Master | 2 | 5% |
| Other | 1 | 3% |
| Unknown | 14 | 36% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 10 | 26% |
| Medicine and Dentistry | 7 | 18% |
| Agricultural and Biological Sciences | 4 | 10% |
| Chemical Engineering | 1 | 3% |
| Social Sciences | 1 | 3% |
| Other | 3 | 8% |
| Unknown | 13 | 33% |