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Timeline
Mendeley readers
Chapter title |
Multiplex Genome Editing in Escherichia coli
|
---|---|
Chapter number | 8 |
Book title |
Synthetic Metabolic Pathways
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7295-1_8 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7294-4, 978-1-4939-7295-1
|
Authors |
Sheila Ingemann Jensen, Alex Toftgaard Nielsen |
Abstract |
Lambda Red recombineering is an easy and efficient method for generating genetic modifications in Escherichia coli. For gene deletions, lambda Red recombineering is combined with the use of selectable markers, which are removed through the action of, e.g., flippase (Flp) recombinase. This PCR-based engineering method has also been applied to a number of other bacteria. In this chapter, we describe a recently developed one plasmid-based method as well as the use of a strain with genomically integrated recombineering genes, which significantly speeds up the engineering of strains with multiple genomic alterations. |
Mendeley readers
The data shown below were compiled from readership statistics for 22 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 22 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 5 | 23% |
Student > Ph. D. Student | 5 | 23% |
Researcher | 2 | 9% |
Other | 1 | 5% |
Student > Doctoral Student | 1 | 5% |
Other | 1 | 5% |
Unknown | 7 | 32% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 5 | 23% |
Biochemistry, Genetics and Molecular Biology | 4 | 18% |
Engineering | 2 | 9% |
Chemical Engineering | 1 | 5% |
Chemistry | 1 | 5% |
Other | 1 | 5% |
Unknown | 8 | 36% |