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Drug Safety Evaluation

Overview of attention for book
Cover of 'Drug Safety Evaluation'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Developing Combination Drugs in Preclinical Studies
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    Chapter 2 Preclinical Evaluation of Juvenile Toxicity
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    Chapter 3 Necropsy and Sampling Procedures in Rodents
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    Chapter 4 Histopathology Procedures: From Tissue Sampling to Histopathological Evaluation
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    Chapter 5 Principles and methods of immunohistochemistry.
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    Chapter 6 Tissue Microarrays and Digital Image Analysis
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    Chapter 7 Micronucleus Assay and Labeling of Centromeres with FISH Technique
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    Chapter 8 The Use of Bacterial Repair Endonucleases in the Comet Assay
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    Chapter 9 Manual Whole-Cell Patch-Clamping of the HERG Cardiac K + Channel
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    Chapter 10 Generation and Analysis of Transcriptomics Data
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    Chapter 11 Protocols of Two-Dimensional Difference Gel Electrophoresis to Investigate Mechanisms of Toxicity
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    Chapter 12 Protocols and Applications of Cellular Metabolomics in Safety Studies Using Precision-Cut Tissue Slices and Carbon 13 NMR
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    Chapter 13 Statistical analysis of quantitative RT-PCR results.
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    Chapter 14 Evaluation of Mitochondrial Respiration in Cultured Rat Hepatocytes
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    Chapter 15 FETAX Assay for Evaluation of Developmental Toxicity
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    Chapter 16 Evaluation of Embryotoxicity Using the Zebrafish Model
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    Chapter 17 Protocols of In Vitro Protein Covalent Binding Studies in Liver
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    Chapter 18 Utilization of MALDI-TOF to Determine Chemical-Protein Adduct Formation In Vitro
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    Chapter 19 Utilization of LC-MS/MS Analyses to Identify Site-Specific Chemical Protein Adducts In Vitro
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    Chapter 20 One-Dimensional Western Blotting Coupled to LC-MS/MS Analysis to Identify Chemical-Adducted Proteins in Rat Urine
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    Chapter 21 Identification of Chemical-Adducted Proteins in Urine by Multi-dimensional Protein Identification Technology (LC/LC–MS/MS)
  23. Altmetric Badge
    Chapter 22 Optimization of SELDI for Biomarker Detection in Plasma
  24. Altmetric Badge
    Chapter 23 Drug Safety Evaluation
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    Chapter 24 NMR and MS methods for metabonomics.
  26. Altmetric Badge
    Chapter 25 Drug Safety Evaluation
Attention for Chapter 5: Principles and methods of immunohistochemistry.
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Chapter title
Principles and methods of immunohistochemistry.
Chapter number 5
Book title
Drug Safety Evaluation
Published in
Methods in molecular biology, October 2010
DOI 10.1007/978-1-60761-849-2_5
Pubmed ID
Book ISBNs
978-1-60327-186-8, 978-1-60761-849-2
Authors

Ramos-Vara JA, José A. Ramos-Vara, Ramos-Vara, José A.

Abstract

Immunohistochemical techniques detect antigens in tissue sections by means of immunological and chemical reactions. This technique is highly sensitive and specific and can detect a wide variety of antigens in multiple animal species. This chapter reviews common immunohistochemical methods used in the characterization of normal and pathologic tissue and the reagents used. Pretreatments such as blocking steps for endogenous activities and antigen retrieval are included. Standard procedures on formalin-fixed, paraffin-embedded tissues as well as method standardization for new antibodies and troubleshooting are emphasized.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 274 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 274 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 20 7%
Student > Ph. D. Student 10 4%
Student > Master 8 3%
Student > Postgraduate 5 2%
Student > Doctoral Student 4 1%
Other 6 2%
Unknown 221 81%
Readers by discipline Count As %
Medicine and Dentistry 20 7%
Biochemistry, Genetics and Molecular Biology 13 5%
Pharmacology, Toxicology and Pharmaceutical Science 4 1%
Immunology and Microbiology 3 1%
Neuroscience 3 1%
Other 8 3%
Unknown 223 81%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 November 2014.
All research outputs
#20,243,777
of 22,771,140 outputs
Outputs from Methods in molecular biology
#9,862
of 13,090 outputs
Outputs of similar age
#94,066
of 99,432 outputs
Outputs of similar age from Methods in molecular biology
#16
of 23 outputs
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We're also able to compare this research output to 23 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.