| Chapter title |
Stable plastid transformation of petunia.
|
|---|---|
| Chapter number | 17 |
| Book title |
Chloroplast Biotechnology
|
| Published in |
Methods in molecular biology, March 2014
|
| DOI | 10.1007/978-1-62703-995-6_17 |
| Pubmed ID | |
| Book ISBNs |
978-1-62703-994-9, 978-1-62703-995-6
|
| Authors |
Avila EM, Day A, Elena Martin Avila, Anil Day |
| Abstract |
Petunia hybrida is a commercial ornamental plant and is also an important model species for genetic analysis and transgenic research. Here we describe the steps required to isolate stable plastid transformants in P. hybrida using the commercial Pink Wave cultivar. Wave cultivars are popular spreading Petunias sold as ground cover and potted plants. Transgenes introduced into P. hybrida plastids exhibit stable expression over many generations. The development of plastid transformation in P. hybrida provides an enabling technology to bring the benefits of plastid engineering, including maternal inheritance and stable expression of performance-enhancing trait genes, to the important floriculture and horticulture industries. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 8 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 4 | 50% |
| Student > Master | 2 | 25% |
| Researcher | 1 | 13% |
| Professor | 1 | 13% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 4 | 50% |
| Biochemistry, Genetics and Molecular Biology | 3 | 38% |
| Environmental Science | 1 | 13% |