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Chloroplast biotechnology

Overview of attention for book
Chloroplast biotechnology
Humana Press

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 The Plastid Genomes of Flowering Plants
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    Chapter 2 Next-Generation Technologies to Determine Plastid Genome Sequences
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    Chapter 3 Plastid Gene Transcription: Promoters and RNA Polymerases
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    Chapter 4 Plastid mRNA Translation
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    Chapter 5 Engineering Chloroplasts for High-Level Foreign Protein Expression
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    Chapter 6 Excision of Plastid Marker Genes Using Directly Repeated DNA Sequences
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    Chapter 7 Fluorescent Labeling and Confocal Microscopic Imaging of Chloroplasts and Non-green Plastids
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    Chapter 8 Plastid Transformation in Nicotiana tabacum and Nicotiana sylvestris by Biolistic DNA Delivery to Leaves
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    Chapter 9 Nicotiana tabacum : PEG-Mediated Plastid Transformation
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    Chapter 10 Plastid Transformation of Tobacco Suspension Cell Cultures
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    Chapter 11 Tryptophan and Indole Analog Mediated Plastid Transformation
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    Chapter 12 Plastid Marker Gene Excision in Greenhouse-Grown Tobacco by Agrobacterium-Delivered Cre Recombinase
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    Chapter 13 Determination of the Half-Life of Chloroplast Transcripts in Tobacco Leaves
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    Chapter 14 Quantification of Organellar DNA and RNA Using Real-Time PCR
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    Chapter 15 Plastid Transformation for Rubisco Engineering and Protocols for Assessing Expression
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    Chapter 16 Plastid Transformation in Tomato
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    Chapter 17 Stable plastid transformation of petunia.
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    Chapter 18 Plastid Transformation in Potato: Solanum tuberosum
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    Chapter 19 Plastid Transformation in Eggplant
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    Chapter 20 Plastid Transformation in Lettuce ( Lactuca sativa L.) by Polyethylene Glycol Treatment of Protoplasts
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    Chapter 21 Plastid Transformation in Lettuce ( Lactuca sativa L.) by Biolistic DNA Delivery
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    Chapter 22 Plastid Transformation in Soybean
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    Chapter 23 Plastid Transformation in Cabbage (Brassica oleracea L. var. capitata L.) by the Biolistic Process.
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    Chapter 24 Plastid Transformation in Sugar Beet: Beta vulgaris
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    Chapter 25 Integration and Expression of gfp in the Plastid of Medicago sativa L.
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    Chapter 26 Rapid Screening for the Robust Expression of Recombinant Proteins in Algal Plastids
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    Chapter 27 A Simple, Low-Cost Method for Chloroplast Transformation of the Green Alga Chlamydomonas reinhardtii
  29. Altmetric Badge
    Chapter 28 Tools for Regulated Gene Expression in the Chloroplast of Chlamydomonas
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    Chapter 29 Plastid Transformation in Physcomitrella patens
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    Chapter 30 Plastid Transformation of Sporelings and Suspension-Cultured Cells from the Liverwort Marchantia polymorpha L.
Attention for Chapter 29: Plastid Transformation in Physcomitrella patens
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Chapter title
Plastid Transformation in Physcomitrella patens
Chapter number 29
Book title
Chloroplast Biotechnology
Published in
Methods in molecular biology, March 2014
DOI 10.1007/978-1-62703-995-6_29
Pubmed ID
Book ISBNs
978-1-62703-994-9, 978-1-62703-995-6
Authors

Sugita M, Mamoru Sugita

Abstract

The moss Physcomitrella patens performs efficient homologous recombination in both the nucleus and plastid enabling the study of individual gene function by generating precise inactivation or modification of genes. Polyethylene glycol (PEG)-mediated transformation of protoplasts is routinely used to study the nuclear gene function of P. patens. PEG-mediated protoplast transformation is also applied for plastid transformation of this moss. The efficiency of plastid transformation is quite reliable, and one or two homoplasmic transplastomic lines are obtained in a plastid transformation experiment (5 × 10(5) protoplasts) by selection for spectinomycin resistance.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 13 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 13 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 3 23%
Student > Bachelor 3 23%
Student > Ph. D. Student 2 15%
Student > Master 2 15%
Lecturer > Senior Lecturer 1 8%
Other 0 0%
Unknown 2 15%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 7 54%
Agricultural and Biological Sciences 3 23%
Unknown 3 23%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 November 2014.
All research outputs
#18,384,336
of 22,771,140 outputs
Outputs from Methods in molecular biology
#7,867
of 13,090 outputs
Outputs of similar age
#160,754
of 220,988 outputs
Outputs of similar age from Methods in molecular biology
#48
of 149 outputs
Altmetric has tracked 22,771,140 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,090 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 220,988 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 14th percentile – i.e., 14% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 149 others from the same source and published within six weeks on either side of this one. This one is in the 42nd percentile – i.e., 42% of its contemporaries scored the same or lower than it.