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Nanoparticles in Biology and Medicine

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Cover of 'Nanoparticles in Biology and Medicine'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Procedures for the Synthesis and Capping of Metal Nanoparticles
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    Chapter 2 Preparation of Concentrated Colloids of Gold Core-Silica Shell Nanoparticles for Biomedical Applications
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    Chapter 3 Biosynthesis of Silver and Gold Nanoparticles Using Bacillus licheniformis
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    Chapter 4 Gold Nanoparticle Antibody Conjugates for Use in Competitive Lateral Flow Assays
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    Chapter 5 Gold Nanoparticles for Immunogold Localization of Antigens in Plant Tissues
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    Chapter 6 RNA Quantification Using Noble Metal Nanoprobes: Simultaneous Identification of Several Different mRNA Targets Using Color Multiplexing and Application to Cancer Diagnostics
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    Chapter 7 Effect of Gold Nanoparticle Conjugation on the Activity and Stability of Functional Proteins
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    Chapter 8 Synthesis of Semiconductor Nanoparticles
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    Chapter 9 Size-Selective Synthesis of Ultrasmall Hydrophilic CdSe Nanoparticles in Aqueous Solution at Room Temperature
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    Chapter 10 Synthesis and Functionalization of CdSe/ZnS QDs Using the Successive Ion Layer Adsorption Reaction and Mercaptopropionic Acid Phase Transfer Methods
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    Chapter 11 In Vivo, Site-Specific, Covalent Conjugation of Quantum Dots to Proteins via Split-Intein Splicing
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    Chapter 12 Evaluating the Potential of Quantum Dots for In Vitro Biological Studies: Effects on Gene Expression Using Microarray Analysis
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    Chapter 13 The Use of Quantum Dots for Immunochemistry Applications
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    Chapter 14 Quantum Dots for Labeling Live Cells
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    Chapter 15 Labeling and Imaging Mesenchymal Stem Cells with Quantum Dots
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    Chapter 16 The Application of Semiconductor Quantum Dots for Enhancing Peptide Desorption, Improving Peak Resolution and Sensitivity of Detection in Matrix-Assisted Laser Desorption/Ionization (MALDI) Mass Spectrometry
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    Chapter 17 Near-Infrared Fluorescence Labeling of Iron Nanoparticles and Applications for Cell Labeling and In Vivo Imaging
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    Chapter 18 Labeling Stem Cells with Superparamagnetic Iron Oxide Nanoparticles: Analysis of the Labeling Efficacy by Microscopy and Magnetic Resonance Imaging
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    Chapter 19 Noninvasive Quantification of Magnetic Nanoparticles by Means of Magnetorelaxometry
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    Chapter 20 Nanoparticles in Biology and Medicine
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    Chapter 21 Synthesis of Biocompatible Gelatinated Thioglycolic Acid-Capped CdTe Quantum Dots (“Jelly Dots”)
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    Chapter 22 Preparation and Characterization of Biomimetic Nanoparticles for Drug Delivery
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    Chapter 23 Preparation of Nanoparticles by Spray-Drying and Their Use for Efficient Pulmonary Drug Delivery
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    Chapter 24 Synthesis and Evaluation of Airway Targeted PLGA Nanoparticles for Drug Delivery in Obstructive Lung Diseases.
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    Chapter 25 Preparation of siRNA-Encapsulated PLGA Nanoparticles for Sustained Release of siRNA and Evaluation of Encapsulation Efficiency
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    Chapter 26 Synthesis of Poly (2-Hydroxyethyl Methacrylate) (PHEMA) Based Nanoparticles for Biomedical and Pharmaceutical Applications
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    Chapter 27 Preparation of Lipid:Peptide:DNA (LPD) Nanoparticles and Their Use for Gene Transfection
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    Chapter 28 Crotamine: A Novel Cell-Penetrating Polypeptide Nanocarrier with Potential Anti-Cancer and Biotechnological Applications
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    Chapter 29 Elastin-based nanoparticles for delivery of bone morphogenetic proteins.
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    Chapter 30 Synthesis, Surface Modification, Characterization, and Biomedical In Vitro Applications of Organically Modified Silica (ORMOSIL) Nanoparticles
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    Chapter 31 Extraction of Organic Nanoparticles from Plants
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    Chapter 32 Assessment of Cytotoxicity of Carbon Nanoparticles Using 3-(4,5-Dimethylthiazol-2-yl)-5-(3-Carboxymethoxyphenyl)-2-(4-Sulfophenyl)-2H-Tetrazolium (MTS) Cell Viability Assay
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    Chapter 33 Nanoparticles in Biology and Medicine
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    Chapter 34 Nanoparticles in Biology and Medicine
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    Chapter 35 Assessment of Toxicity of Nanoparticles Using Insects as Biological Models
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    Chapter 36 Evaluation of Cytotoxicity of 3-Mercaptopropionic Acid-Modified Quantum Dots on Medicago sativa Cells and Tissues
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    Chapter 37 Scanning Transmission Electron Microscopy Methods for the Analysis of Nanoparticles
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    Chapter 38 Nanoparticles in Biology and Medicine
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    Chapter 39 Microscopy Imaging Methods for the Detection of Silver and Titanium Nanoparticles Within Cells
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    Chapter 40 Detection of TiO 2 Nanoparticles in Cells by Flow Cytometry
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    Chapter 41 Nanoparticle Tracking Analysis for the Multiparameter Characterization and Counting of Nanoparticle Suspensions
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    Chapter 42 Relevance of Two-Dimensional Brownian Motion Dynamics in Applying Nanoparticle Tracking Analysis
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    Chapter 43 Nanoparticle Bridges for Studying Electrical Properties of Organic Molecules
Attention for Chapter 29: Elastin-based nanoparticles for delivery of bone morphogenetic proteins.
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Chapter title
Elastin-based nanoparticles for delivery of bone morphogenetic proteins.
Chapter number 29
Book title
Nanoparticles in Biology and Medicine
Published in
Methods in molecular biology, July 2012
DOI 10.1007/978-1-61779-953-2_29
Pubmed ID
Book ISBNs
978-1-61779-952-5, 978-1-61779-953-2
Authors

Machado R, Bessa PC, Reis RL, Rodriguez-Cabello JC, Casal M, Raul Machado, Paulo C. Bessa, Rui L. Reis, José Carlos Rodriguez-Cabello, Margarida Casal

Abstract

Biocompatibility, stability, and efficiency remain among the major goals in the development of new drug delivery systems. Herein we describe a facile method for encapsulation of Bone Morphogenetic Protein-2 (BMP-2) at high concentrations and in mild conditions by exploiting the thermo-responsiveness of an elastin-like polymer. Chemically synthesized poly(VPAVG) demonstrates excellent biocompatibility, stability at room temperature, and allows the use of mild conditions and harmless solvents, such as water, for its production. Here, we describe how to use a recombinantly produced poly(VPAVG) polymer to entrap BMP-2 with a high encapsulation efficiency and the methods for in vitro bioactivity assays.

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The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 23 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 23 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 4 17%
Professor 3 13%
Student > Master 3 13%
Student > Ph. D. Student 3 13%
Student > Doctoral Student 2 9%
Other 2 9%
Unknown 6 26%
Readers by discipline Count As %
Agricultural and Biological Sciences 6 26%
Materials Science 4 17%
Biochemistry, Genetics and Molecular Biology 2 9%
Medicine and Dentistry 2 9%
Unspecified 1 4%
Other 2 9%
Unknown 6 26%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 November 2014.
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#20,242,779
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Outputs from Methods in molecular biology
#9,862
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Outputs of similar age
#148,228
of 164,647 outputs
Outputs of similar age from Methods in molecular biology
#50
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So far Altmetric has tracked 13,090 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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