Chapter title |
Liver Bioengineering Using Decellularized Whole-Liver Scaffolds
|
---|---|
Chapter number | 98 |
Book title |
Decellularized Scaffolds and Organogenesis
|
Published in |
Methods in molecular biology, November 2017
|
DOI | 10.1007/7651_2017_98 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7655-3, 978-1-4939-7656-0
|
Authors |
Iris Pla-Palacín, Pilar Sainz-Arnal, Sara Morini, Manuel Almeida, Pedro M. Baptista, Pla-Palacín, Iris, Sainz-Arnal, Pilar, Morini, Sara, Almeida, Manuel, Baptista, Pedro M. |
Abstract |
Currently, due to the progress made in the field of regenerative medicine, whole-organ bioengineering is becoming a valid alternative to cope with the shortages of organs for transplantation. In this chapter, we describe the main techniques carried out for pig liver bioengineering, which serves as an essential model for future human liver bioengineering. These include porcine whole-liver decellularization, endothelial and mesenchymal stem cell isolation, porcine ES-derived hepatoblasts, and scaffold recellularization using a bioreactor perfusion system. |
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