| Chapter title |
Simultaneous Detection of Autophagy and Epithelial to Mesenchymal Transition in the Non-small Cell Lung Cancer Cells
|
|---|---|
| Chapter number | 84 |
| Book title |
Autophagy in Differentiation and Tissue Maintenance
|
| Published in |
Methods in molecular biology, November 2017
|
| DOI | 10.1007/7651_2017_84 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-8747-4, 978-1-4939-8748-1
|
| Authors |
Javad Alizadeh, Shahla Shojaei, Adel Sepanjnia, Mohammad Hashemi, Eftekhar Eftekharpour, Saeid Ghavami, Alizadeh, Javad, Shojaei, Shahla, Sepanjnia, Adel, Hashemi, Mohammad, Eftekharpour, Eftekhar, Ghavami, Saeid |
| Abstract |
Autophagy is increasingly identified as a central player in many cellular activities from cell proliferation to cell division, migration, and differentiation. However, it is also considered as a double-edged sword in cancer biology which either promotes oncogenesis/invasion or sensitizes the tumor cells to chemotherapy induced apoptosis. Recent investigations have provided direct evidence for regulation of cellular phenotype via autophagy pathway. One of the most important types of phenotype conversion is Epithelial-Mesenchymal-Transition (EMT), resulting in alteration of epithelial cell properties to a more mesenchymal form. In the current chapter, we provide a method which is established and being used in our laboratory for detection of autophagy and EMT in lung epithelial cells and show the involvement of autophagy in modulation of cellular phenotype. |
X Demographics
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|---|---|---|
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| Unknown | 1 | 25% |
Demographic breakdown
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|---|---|---|
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| Scientists | 2 | 50% |
Mendeley readers
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|---|---|---|
| Unknown | 14 | 100% |
Demographic breakdown
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|---|---|---|
| Other | 2 | 14% |
| Student > Doctoral Student | 2 | 14% |
| Professor | 2 | 14% |
| Student > Ph. D. Student | 2 | 14% |
| Student > Master | 1 | 7% |
| Other | 1 | 7% |
| Unknown | 4 | 29% |
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|---|---|---|
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| Energy | 1 | 7% |
| Neuroscience | 1 | 7% |
| Other | 2 | 14% |
| Unknown | 5 | 36% |